Human PALM ELISA Kit

Alternative name
Human paralemmin ELISA Kit;Human KIAA0270 ELISA Kit;Human paralemmin-1 ELISA Kit;
Catalog
E028282
species
Human
GenePALM
SpecificityThis assay has high sensitivity and excellent specificity for detection of paralemmin. No significant cross-reactivity or interference between paralemmin and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of paralemmin is typically less than 0.057ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addi
Intended UseHuman PALM ELISA Kit allows for the in vitro quantitative determination of PALM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to paralemmin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to paralemmin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain paralemmin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of paralemmin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Paralemmin-1 Protein information
Uniprot ID PALM_HUMAN
Uniprot AC O75781; O43359; O95673; Q92559; Q9UPJ4; Q9UQS2; Q9UQS3;
UniGene Hs.631841;
GeneID 5064
KEGG hsa:5064;
Human Paralemmin-1 Protein SEQUENCE
SEQUENCE 387 AA; 42076 MW; 46D455D5D12C3135 CRC64;
MEVLAAETTS QQERLQAIAE KRKRQAEIEN KRRQLEDERR QLQHLKSKAL
RERWLLEGTP SSASEGDEDL RRQMQDDEQK TRLLEDSVSR LEKEIEVLER
GDSAPATAKE NAAAPSPVRA PAPSPAKEER KTEVVMNSQQ TPVGTPKDKR
VSNTPLRTVD GSPMMKAAMY SVEITVEKDK VTGETRVLSS TTLLPRQPLP
LGIKVYEDET KVVHAVDGTA ENGIHPLSSS EVDELIHKAD EVTLSEAGST
AGAAETRGAV EGAARTTPSR REITGVQAQP GEATSGPPGI QPGQEPPVTM
IFMGYQNVED EAETKKVLGL QDTITAELVV IEDAAEPKEP APPNGSAAEP
PTEAASREEN QAGPEATTSD PQDLDMKKHR CKCCSIM
UCSC uc002lpm.2; human. [O75781-1];

Category

Human PALM ELISA Kit

Human paralemmin ELISA Kit;Human KIAA0270 ELISA Kit;Human paralemmin-1 ELISA Kit;