Human PNLIP ELISA Kit

Alternative name
Human PL ELISA Kit;Human PTL ELISA Kit;Human pancreatic lipase ELISA Kit;Human PNLIPD ELISA Kit;Human pancreatic triacylglycerol lipase ELISA Kit;Human triacylglycerol acylhydrolase ELISA Kit;
Catalog
E028183
species
Human
GenePNLIP
SpecificityThis assay has high sensitivity and excellent specificity for detection of human PL. No significant cross-reactivity or interference between human PL and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human PL is typically less than 0.156 U/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
Intended UseHuman PNLIP ELISA Kit allows for the in vitro quantitative determination of PNLIP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Pancreatic triacylglycerol lipase Protein information
Uniprot ID LIPP_HUMAN
Uniprot AC P16233; Q5VSQ2;
UniGene Hs.501135;
GeneID 5406
KEGG hsa:5406;
Human Pancreatic triacylglycerol lipase Protein SEQUENCE
SEQUENCE 465 AA; 51157 MW; 2BC49CC7F0E2DF52 CRC64;
MLPLWTLSLL LGAVAGKEVC YERLGCFSDD SPWSGITERP LHILPWSPKD
VNTRFLLYTN ENPNNFQEVA ADSSSISGSN FKTNRKTRFI IHGFIDKGEE
NWLANVCKNL FKVESVNCIC VDWKGGSRTG YTQASQNIRI VGAEVAYFVE
FLQSAFGYSP SNVHVIGHSL GAHAAGEAGR RTNGTIGRIT GLDPAEPCFQ
GTPELVRLDP SDAKFVDVIH TDGAPIVPNL GFGMSQVVGH LDFFPNGGVE
MPGCKKNILS QIVDIDGIWE GTRDFAACNH LRSYKYYTDS IVNPDGFAGF
PCASYNVFTA NKCFPCPSGG CPQMGHYADR YPGKTNDVGQ KFYLDTGDAS
NFARWRYKVS VTLSGKKVTG HILVSLFGNK GNSKQYEIFK GTLKPDSTHS
NEFDSDVDVG DLQMVKFIWY NNVINPTLPR VGASKIIVET NVGKQFNFCS
PETVREEVLL TLTPC
UCSC uc001lcm.4; human.;

Category

Human PNLIP ELISA Kit

Human PL ELISA Kit;Human PTL ELISA Kit;Human pancreatic lipase ELISA Kit;Human PNLIPD ELISA Kit;Human pancreatic triacylglycerol lipase ELISA Kit;Human triacylglycerol acylhydrolase ELISA Kit;