Human PAX9 ELISA Kit

Contact Us
  • Alternative name

    Human STHAG3 ELISA Kit;Human paired box 9 ELISA Kit;Human paired box protein Pax-9 ELISA Kit;Human paired domain gene 9 ELISA Kit;

  • Catalog
    E028126
  • species
    Human
  • GenePAX9
  • Standard CurveHuman PAX9 ELISA Kit
  • Other Species Mouse Pax9 ELISA KitChicken PAX9 ELISA KitRat Pax9 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman PAX9 ELISA Kit allows for the in vitro quantitative determination of PAX9 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: PAX9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PAX9 antibody and an PAX9-HRP conjugate. The assay sample and buffer are incubated together with PAX9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PAX9 concentration since PAX9 from samples and PAX9-HRP conjugate compete for the anti-PAX9 antibody binding site. Since the number of sites is limited, as more sites are occupied by PAX9 from the sample, fewer sites are left to bind PAX9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAX9 concentration in each sample is interpolated from this standard curve.
  • Human Paired box protein Pax-9 Protein information
  • Uniprot ID PAX9_HUMAN
  • Uniprot AC P55771; Q99582; Q9UQR4;
  • UniGene Hs.132576; Hs.609574;
  • GeneID 5083
  • KEGG hsa:5083;
  • Human Paired box protein Pax-9 Protein SEQUENCE
  • SEQUENCE 341 AA; 36310 MW; F5E6B0BC991E7C1D CRC64;

    MEPAFGEVNQ LGGVFVNGRP LPNAIRLRIV ELAQLGIRPC DISRQLRVSH

    GCVSKILARY NETGSILPGA IGGSKPRVTT PTVVKHIRTY KQRDPGIFAW

    EIRDRLLADG VCDKYNVPSV SSISRILRNK IGNLAQQGHY DSYKQHQPTP

    QPALPYNHIY SYPSPITAAA AKVPTPPGVP AIPGSVAMPR TWPSSHSVTD

    ILGIRSITDQ VSDSSPYHSP KVEEWSSLGR NNFPAAAPHA VNGLEKGALE

    QEAKYGQAPN GLPAVGSFVS ASSMAPYPTP AQVSPYMTYS AAPSGYVAGH

    GWQHAGGTSL SPHNCDIPAS LAFKGMQAAR EGSHSVTASA L




Inquiry About Human PAX9 ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!