Human SERPINE1 ELISA Kit

Alternative name
Human PAI ELISA Kit;Human PAI-1 ELISA Kit;Human endothelial plasminogen activator inhibitor ELISA Kit;Human serpin E1 ELISA Kit;Human PAI1 ELISA Kit;Human PLANH1 ELISA Kit;Human serpin family E member 1 ELISA Kit;Human plasminogen activator inhibitor 1 ELISA Kit;Human serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 ELISA Kit;Human serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 ELISA Kit;
Catalog
E028111
species
Human
GeneSERPINE1
Standard Curve
Other Species Mouse Serpine1 ELISA Kit Bovine SERPINE1 ELISA Kit Porcine SERPINE1 ELISA Kit Rat Serpine1 ELISA Kit
SpecificityNatural and recombinant human PAI-1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman SERPINE1 ELISA Kit allows for the in vitro quantitative determination of SERPINE1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human PAI-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PAI-1 has been precoated onto 96-well plates. Standards(sf21, S22-P402) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PAI-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PAI-1 amount of sample captured in plate. Background: Plasminogen activator inhibitor-1 is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), the activators of plasminogen and hence fibrinolysis (the physiological breakdown of blood clots). It is a serine protease inhibitor (serpin) protein (SERPINE1). PAI-1 is mainly produced by the endothelium (cells lining blood vessels), but is also secreted by other tissue types, such as adipose tissue. The PAI-1 gene is located on the seventh chromosome (7q21.3-q22).1 Congenital deficiency of PAI-1 leads to a hemorrhagic diathesis (a tendency tohemorrhage). PAI-1 is present in increased levels in various disease states (such as a number of forms of cancer), as well as in obesity and the metabolic syndrome. It has been linked to the increased occurrence of thrombosis in patients with these conditions. Additionally, it has been shown to interact with ORM1.2 The standard product used inthis kit is recombinant human PAI-1 with the molecular mass of 43KDa.
Human Plasminogen activator inhibitor 1 Protein information
Uniprot ID PAI1_HUMAN
Uniprot AC P05121; B7Z4S0; F8WD53;
UniGene Hs.414795; Hs.713079; Hs.741440;
GeneID 5054
KEGG hsa:5054;
Human Plasminogen activator inhibitor 1 Protein SEQUENCE
SEQUENCE 402 AA; 45060 MW; A2E181ED28DD6082 CRC64;
MQMSPALTCL VLGLALVFGE GSAVHHPPSY VAHLASDFGV RVFQQVAQAS
KDRNVVFSPY GVASVLAMLQ LTTGGETQQQ IQAAMGFKID DKGMAPALRH
LYKELMGPWN KDEISTTDAI FVQRDLKLVQ GFMPHFFRLF RSTVKQVDFS
EVERARFIIN DWVKTHTKGM ISNLLGKGAV DQLTRLVLVN ALYFNGQWKT
PFPDSSTHRR LFHKSDGSTV SVPMMAQTNK FNYTEFTTPD GHYYDILELP
YHGDTLSMFI AAPYEKEVPL SALTNILSAQ LISHWKGNMT RLPRLLVLPK
FSLETEVDLR KPLENLGMTD MFRQFQADFT SLSDQEPLHV AQALQKVKIE
VNESGTVASS STAVIVSARM APEEIIMDRP FLFVVRHNPT GTVLFMGQVM
EP
UCSC uc003uxt.4; human. [P05121-1];

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Human SERPINE1 ELISA Kit