Human PACAP-27 ELISA Kit

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  • Alternative name

    Pituitary adenylate cyclase-activating polypeptide type I receptor ELISA KIT; ADCYAP1R1 ELISA KIT; PACAP type I receptor ELISA KIT; PACAP-R-1 ELISA KIT; PACAP-R1 ELISA KIT

  • Catalog
  • species
  • GenePACAP-27
  • Other Species Mouse PACAP-27 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human PACAP-27. No significant cross-reactivity or interference between Human PACAP-27 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human PACAP-27 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman PACAP-27 ELISA Kit allows for the in vitro quantitative determination of PACAP-27 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PACAP-27 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PACAP-27. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PACAP-27 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PACAP-27, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PACAP-27. You can calculate the concentration of Human PACAP-27 in the samples by comparing the OD of the samples to the standard curve.

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