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Human P2RY2 ELISA Kit

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  • Alternative name

    Human P2Y2 ELISA Kit;Human ATP receptor ELISA Kit;Human P2U purinoceptor 1 ELISA Kit;Human P2U1 ELISA Kit;Human P2U receptor 1 ELISA Kit;Human Purinergic receptor ELISA Kit;Human P2RU1 ELISA Kit;Human HP2U ELISA Kit;Human P2U ELISA Kit;Human P2UR ELISA Kit;Human P2Y2R ELISA Kit;Human purinergic receptor P2Y2 ELISA Kit;Human P2Y purinoceptor 2 ELISA Kit;Human P2U nucleotide receptor ELISA Kit;Human purinergic receptor P2Y, G-protein coupled, 2 ELISA Kit;Human purinoceptor P2Y2 ELISA Kit;
  • Catalog
    E028080
  • species
    Human
  • GeneP2RY2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of P2RY2. No significant cross-reactivity or interference between P2RY2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between P2RY2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman P2RY2 ELISA Kit allows for the in vitro quantitative determination of P2RY2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical    Principle of the assay: P2RY2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-P2RY2 antibody and an P2RY2-HRP conjugate. The assay sample and buffer are incubated together with P2RY2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the P2RY2 concentration since P2RY2 from samples and P2RY2-HRP conjugate compete for the anti-P2RY2 antibody binding site. Since the number of sites is limited, as more sites are occupied by P2RY2 from the sample, fewer sites are left to bind P2RY2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The P2RY2 concentration in each sample is interpolated from this standard curve.
  • Human P2Y purinoceptor 2 Protein information
  • Uniprot ID P2RY2_HUMAN
  • Uniprot AC P41231; B2R9W3; Q96EM8;
  • UniGene Hs.339; Hs.572077;
  • GeneID 5029
  • KEGG hsa:5029;
  • Human P2Y purinoceptor 2 Protein SEQUENCE

  • SEQUENCE 377 AA; 42273 MW; F71DAF4A8DCB4062 CRC64;

    MAADLGPWND TINGTWDGDE LGYRCRFNED FKYVLLPVSY GVVCVPGLCL

    NAVALYIFLC RLKTWNASTT YMFHLAVSDA LYAASLPLLV YYYARGDHWP

    FSTVLCKLVR FLFYTNLYCS ILFLTCISVH RCLGVLRPLR SLRWGRARYA

    RRVAGAVWVL VLACQAPVLY FVTTSARGGR VTCHDTSAPE LFSRFVAYSS

    VMLGLLFAVP FAVILVCYVL MARRLLKPAY GTSGGLPRAK RKSVRTIAVV

    LAVFALCFLP FHVTRTLYYS FRSLDLSCHT LNAINMAYKV TRPLASANSC

    LDPVLYFLAG QRLVRFARDA KPPTGPSPAT PARRRLGLRR SDRTDMQRIE

    DVLGSSEDSR RTESTPAGSE NTKDIRL

  • UCSC uc001otj.5; human.;



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