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Human PAK4 ELISA Kit

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  • Alternative name

    Human p21-activated kinase 4 ELISA Kit;Human PAK-4 ELISA Kit;Human KIAA1142 ELISA Kit;Human p21 (RAC1) activated kinase 4 ELISA Kit;Human serine/threonine-protein kinase PAK 4 ELISA Kit;Human p21 protein (Cdc42/Rac)-activated kinase 4 ELISA Kit;Human p21(CDKN1A)-activated kinase 4 ELISA Kit;Human protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs ELISA Kit;
  • Catalog
    E028069
  • species
    Human
  • GenePAK4
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of p21 Protein Activated Kinase 4 (PAK4). No significant cross-reactivity or interference between p21 Protein Activated Kinase 4 (PAK4) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.065ng/mL.
  • Intended UseHuman PAK4 ELISA Kit allows for the in vitro quantitative determination of PAK4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to p21 Protein Activated Kinase 4 (PAK4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to p21 Protein Activated Kinase 4 (PAK4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain p21 Protein Activated Kinase 4 (PAK4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of p21 Protein Activated Kinase 4 (PAK4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Serine/threonine-protein kinase PAK 4 Protein information
  • Uniprot ID PAK4_HUMAN
  • Uniprot AC O96013; B4DGG6; Q8N4E1; Q8NCH5; Q8NDE3; Q9BU33; Q9ULS8;
  • UniGene Hs.20447;
  • GeneID 10298
  • KEGG hsa:10298;
  • Human Serine/threonine-protein kinase PAK 4 Protein SEQUENCE

  • SEQUENCE 591 AA; 64072 MW; 04C2A5C0B06427D5 CRC64;

    MFGKRKKRVE ISAPSNFEHR VHTGFDQHEQ KFTGLPRQWQ SLIEESARRP

    KPLVDPACIT SIQPGAPKTI VRGSKGAKDG ALTLLLDEFE NMSVTRSNSL

    RRDSPPPPAR ARQENGMPEE PATTARGGPG KAGSRGRFAG HSEAGGGSGD

    RRRAGPEKRP KSSREGSGGP QESSRDKRPL SGPDVGTPQP AGLASGAKLA

    AGRPFNTYPR ADTDHPSRGA QGEPHDVAPN GPSAGGLAIP QSSSSSSRPP

    TRARGAPSPG VLGPHASEPQ LAPPACTPAA PAVPGPPGPR SPQREPQRVS

    HEQFRAALQL VVDPGDPRSY LDNFIKIGEG STGIVCIATV RSSGKLVAVK

    KMDLRKQQRR ELLFNEVVIM RDYQHENVVE MYNSYLVGDE LWVVMEFLEG

    GALTDIVTHT RMNEEQIAAV CLAVLQALSV LHAQGVIHRD IKSDSILLTH

    DGRVKLSDFG FCAQVSKEVP RRKSLVGTPY WMAPELISRL PYGPEVDIWS

    LGIMVIEMVD GEPPYFNEPP LKAMKMIRDN LPPRLKNLHK VSPSLKGFLD

    RLLVRDPAQR ATAAELLKHP FLAKAGPPAS IVPLMRQNRT R

  • UCSC uc002okj.2; human. [O96013-1];



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