Human P selectin ELISA Kit

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  • Alternative name

    P-selectin ELISA KIT; CD62 antigen-like family member P ELISA KIT; Granule membrane protein 140 ELISA KIT; GMP-140 ELISA KIT; Leukocyte-endothelial cell adhesion molecule 3 ELISA KIT; LECAM3 ELISA KIT; Platelet activation dependent granule-external membrane protein ELISA KIT; PADGEM ELISA KIT; CD_antigen: CD62PSELP ELISA KIT; GMRP ELISA KIT; GRMP ELISA KIT; GMP-140 ELISA KIT; LECAM3 ELISA KIT; PADGEM ELISA KIT

  • Catalog
  • species
  • GeneP selectin
  • Standard CurveHuman P selectin ELISA Kit
  • Other Species Human P-Selectin/CD62P ELISA KitMouse P-SELECTIN ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman P selectin ELISA Kit allows for the in vitro quantitative determination of P selectin , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology
  • Product Description
    Principle of the Assay: P SELECTIN ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for P SELECTIN. Standards or samples are then added to the microtiter plate wells and P SELECTIN if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of P SELECTIN present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for P SELECTIN are added to each well to "sandwich" the P SELECTIN immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain P SELECTIN and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The P SELECTIN concentration in each sample is interpolated from this standard curve.

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