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Catalog
E027813
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species
Human
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GeneOFQ/N
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Standard Curve
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SpecificitySensitivity: The sensitivity in this assay is 0.1 ng/ml
Specificity: This assay has high sensitivity and excellent specificity for detection of OFQ/N. No significant cross-reactivity or interference between OFQ/N and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseHuman OFQ/N ELISA Kit allows for the in vitro quantitative determination of OFQ/N , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyHuman ELISA Kit
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Product Description
specificalFor Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma
Intended Uses: This OFQ/N ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of OFQ/N in the sample, this OFQ/N ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus OFQ/N concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of OFQ/N utilizes a multiclonal anti-OFQ/N antibody and an OFQ/N-HRP conjugate. The assay sample and buffer are incubated together with OFQ/N-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OFQ/N concentration since OFQ/N from samples and OFQ/N-HRP conjugate compete for the anti-OFQ/N antibody binding site. Since the number of sites is limited, as more sites are occupied by OFQ/N from the sample, fewer sites are left to bind OFQ/N-HRP conjugate. Standards of known OFQ/N concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of OFQ/N. The OFQ/N concentration in each sample is interpolated from this standard curve.
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