-
Alternative name
Orotate phosphoribosyltransferase ELISA KIT; pyrE ELISA KIT; STM3733 ELISA KIT; OPRT ELISA KIT; OPRTase ELISA KIT
-
Catalog
E027809
-
species
Human
-
GeneOPRT
-
SpecificityThis assay has high sensitivity and excellent specificity for detection of human OPRT. No significant cross-reactivity or interference between human OPRT and analogues was observed.
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
SensitivityThe minimum detectable dose of human OPRT is typically less than 11.75 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
-
Intended UseHuman OPRT ELISA Kit allows for the in vitro quantitative determination of OPRT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
-
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for OPRT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any OPRT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OPRT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OPRT bound in the initial step. The color development is stopped and the intensity of the color is measured.
-