Human OPTN ELISA Kit

Alternative name
Human E3-14.7K-interacting protein ELISA Kit;Human FIP-2 ELISA Kit;Human huntingtin yeast partner L ELISA Kit;Human huntingtin-interacting protein 7 ELISA Kit;Human HIP-7 ELISA Kit;Human huntingtin-interacting protein L ELISA Kit;Human nemo-related protein ELISA Kit;Human optic neuropathy-inducing protein ELISA Kit;Human transcription factor IIIA-interacting protein ELISA Kit;Human TFIIIA-INTP ELISA Kit;Human FIP2 ELISA Kit;Human GLC1E ELISA Kit;Human HIP7 ELISA Kit;Human HYPL ELISA Kit;Human NRP ELISA Kit;Human ALS12 ELISA Kit;Human optineurin ELISA Kit;Human Huntingtin interacting protein L ELISA Kit;Human transcrption factor IIIA-interacting protein ELISA Kit;Human tumor necrosis factor alpha-inducible cellular protein containing leucine zipper domains ELISA Kit;
Catalog
E027741
species
Human
GeneOPTN
SpecificityThis assay has high sensitivity and excellent specificity for detection of optineurin. No significant cross-reactivity or interference between optineurin and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of optineurin is typically less than 0.061ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addi
Intended UseHuman OPTN ELISA Kit allows for the in vitro quantitative determination of OPTN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to optineurin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to optineurin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain optineurin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of optineurin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Optineurin Protein information
Uniprot ID OPTN_HUMAN
Uniprot AC Q7LDL9; Q8N562; Q9UET9; Q9UEV4; Q9Y218;
UniGene Hs.332706;
GeneID 10133
KEGG hsa:10133;
Human Optineurin Protein SEQUENCE
SEQUENCE 577 AA; 65921 MW; DB0F841E3315AAE1 CRC64;
MSHQPLSCLT EKEDSPSEST GNGPPHLAHP NLDTFTPEEL LQQMKELLTE
NHQLKEAMKL NNQAMKGRFE ELSAWTEKQK EERQFFEIQS KEAKERLMAL
SHENEKLKEE LGKLKGKSER SSEDPTDDSR LPRAEAEQEK DQLRTQVVRL
QAEKADLLGI VSELQLKLNS SGSSEDSFVE IRMAEGEAEG SVKEIKHSPG
PTRTVSTGTA LSKYRSRSAD GAKNYFEHEE LTVSQLLLCL REGNQKVERL
EVALKEAKER VSDFEKKTSN RSEIETQTEG STEKENDEEK GPETVGSEVE
ALNLQVTSLF KELQEAHTKL SKAELMKKRL QEKCQALERK NSAIPSELNE
KQELVYTNKK LELQVESMLS EIKMEQAKTE DEKSKLTVLQ MTHNKLLQEH
NNALKTIEEL TRKESEKVDR AVLKELSEKL ELAEKALASK QLQMDEMKQT
IAKQEEDLET MTILRAQMEV YCSDFHAERA AREKIHEEKE QLALQLAVLL
KENDAFEDGG RQSLMEMQSR HGARTSDSDQ QAYLVQRGAE DRDWRQQRNI
PIHSCPKCGE VLPDIDTLQI HVMDCII
UCSC uc001ilv.2; human. [Q96CV9-1];

Category

Human OPTN ELISA Kit