Human OPTC ELISA Kit

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  • Alternative name

    Human oculoglycan ELISA Kit;Human OPT ELISA Kit;Human opticin ELISA Kit;

  • Catalog
    E027739
  • species
    Human
  • GeneOPTC
  • Standard CurveHuman OPTC ELISA Kit
  • Other Species Mouse Optc ELISA KitCanine OPTC ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of opticin. No significant cross-reactivity or interference between opticin and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of opticin is typically less than 0.115ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding
  • Intended UseHuman OPTC ELISA Kit allows for the in vitro quantitative determination of OPTC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to opticin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to opticin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain opticin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of opticin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Opticin Protein information
  • Uniprot ID OPT_HUMAN
  • Uniprot AC Q9UBM4; Q5T2G4;
  • UniGene Hs.632468;
  • GeneID 26254
  • KEGG hsa:26254;
  • Human Opticin Protein SEQUENCE
  • SEQUENCE 332 AA; 37261 MW; 8060B4C46DF41C20 CRC64;

    MRLLAFLSLL ALVLQETGTA SLPRKERKRR EEQMPREGDS FEVLPLRNDV

    LNPDNYGEVI DLSNYEELTD YGDQLPEVKV TSLAPATSIS PAKSTTAPGT

    PSSNPTMTRP TTAGLLLSSQ PNHGLPTCLV CVCLGSSVYC DDIDLEDIPP

    LPRRTAYLYA RFNRISRIRA EDFKGLTKLK RIDLSNNLIS SIDNDAFRLL

    HALQDLILPE NQLEALPVLP SGIEFLDVRL NRLQSSGIQP AAFRAMEKLQ

    FLYLSDNLLD SIPGPLPLSL RSVHLQNNLI ETMQRDVFCD PEEHKHTRRQ

    LEDIRLDGNP INLSLFPSAY FCLPRLPIGR FT

  • UCSC uc001gzu.2; human.;


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