Human SPP1 ELISA Kit

Alternative name
Human Bone sialoprotein 1 ELISA Kit;Human nephropontin ELISA Kit;Human secreted phosphoprotein 1 ELISA Kit;Human SPP-1 ELISA Kit;Human urinary stone protein ELISA Kit;Human uropontin ELISA Kit;Human BNSP ELISA Kit;Human OPN ELISA Kit;Human BSPI ELISA Kit;Human ETA-1 ELISA Kit;Human osteopontin ELISA Kit;Human SPP1/CALPHA1 fusion ELISA Kit;Human early T-lymphocyte activation 1 ELISA Kit;Human osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein ELISA Kit;Human secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) ELISA Kit;Human secreted phosphoprotein 1 variant 6 ELISA Kit;
Catalog
E027724
species
Human
GeneSPP1
Standard Curve
Other Species Humanman SPP1 ELISA Kit Mouse Spp1 ELISA Kit Bovine SPP1 ELISA Kit Chicken SPP1 ELISA Kit Porcine SPP1 ELISA Kit Rabbit SPP1 ELISA Kit Rat Spp1 ELISA Kit Sheep SPP1 ELISA Kit
SpecificityNatural and recombinant human OPN
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman SPP1 ELISA Kit allows for the in vitro quantitative determination of SPP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human OPN ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for OPN has been precoated onto 96-well plates. Standards(NSO,I17-N300) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for OPN is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human OPN amount of sample captured in plate. Background: Osteopontin (OPN) also called urinary stone protein, secreted phosphoprotein 1 (SPP1), bone sialoprotein, and early T lymphocyte activation 1 (ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extracellular matrix by osteoblasts during bone development. It is believed to facilitate the attachment of osteoblasts and osteoclasts to the extracellular matrix, allowing them to perform their respective functions during osteogenesis.1Osteopontin is presumably involved in stone formation as stone matrix.2 The deduced protein sequence reveals a317-amino acid protein (34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4.3 The standard product used in this kit is recombinant human OPN, consistingof 17-314 amino acid sequence with the molecular mass of 32.9KDa. As a result of glycosylation, the molecular mass is 60-65KDa.
Human Osteopontin Protein information
Uniprot ID OSTP_HUMAN
Uniprot AC Q96IZ1;
UniGene Hs.313;
GeneID 6696
KEGG hsa:6696;
Human Osteopontin Protein SEQUENCE
SEQUENCE 314 AA; 35423 MW; 4996429EC4752B86 CRC64;
MRIAVICFCL LGITCAIPVK QADSGSSEEK QLYNKYPDAV ATWLNPDPSQ
KQNLLAPQNA VSSEETNDFK QETLPSKSNE SHDHMDDMDD EDDDDHVDSQ
DSIDSNDSDD VDDTDDSHQS DESHHSDESD ELVTDFPTDL PATEVFTPVV
PTVDTYDGRG DSVVYGLRSK SKKFRRPDIQ YPDATDEDIT SHMESEELNG
AYKAIPVAQD LNAPSDWDSR GKDSYETSQL DDQSAETHSH KQSRLYKRKA
NDESNEHSDV IDSQELSKVS REFHSHEFHS HEDMLVVDPK SKEEDKHLKF
RISHELDSAS SEVN
UCSC uc003hra.4; human. [P10451-1];

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Human SPP1 ELISA Kit