Human opiorphin ELISA Kit

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  • Catalog
    E027721
  • species
    Human
  • Geneopiorphin
  • Standard CurveHuman opiorphin ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.156 ng/ml
  • Intended UseHuman opiorphin ELISA Kit allows for the in vitro quantitative determination of opiorphin , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for opiorphin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any opiorphin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for opiorphin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of opiorphin bound in the initial step. The color development is stopped and the intensity of the color is measured.



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