Human OR7D4 ELISA Kit

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  • Alternative name

    Human OR19-B ELISA Kit;Human Odorant receptor family subfamily D member 4RT ELISA Kit;Human olfactory receptor OR19-7 ELISA Kit;Human OR7D4P ELISA Kit;Human OR19-7 ELISA Kit;Human OR19B ELISA Kit;Human hg105 ELISA Kit;Human olfactory receptor family 7 subfamily D member 4 ELISA Kit;Human olfactory receptor 7D4 ELISA Kit;Human odorant receptor family 7 subfamily D member 4 RT ELISA Kit;Human olfactory receptor, family 7, subfamily D, member 4 pseudogene ELISA Kit;

  • Catalog
    E027609
  • species
    Human
  • GeneOR7D4
  • Standard CurveHuman OR7D4 ELISA Kit
  • Other Species Mouse OR7D4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of OR7D4. No significant cross-reactivity or interference between OR7D4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between OR7D4 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman OR7D4 ELISA Kit allows for the in vitro quantitative determination of OR7D4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical
    Principle of the assay: OR7D4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OR7D4 antibody and an OR7D4-HRP conjugate. The assay sample and buffer are incubated together with OR7D4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OR7D4 concentration since OR7D4 from samples and OR7D4-HRP conjugate compete for the anti-OR7D4 antibody binding site. Since the number of sites is limited, as more sites are occupied by OR7D4 from the sample, fewer sites are left to bind OR7D4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OR7D4 concentration in each sample is interpolated from this standard curve.
  • Human Olfactory receptor 7D4 Protein information
  • Uniprot ID OR7D4_HUMAN
  • Uniprot AC Q8NG98; A8CAH8; A8CAH9; A8CAI0; A8CAI1; B9EH79;
  • UniGene Hs.446910;
  • GeneID 125958
  • KEGG hsa:125958;
  • Human Olfactory receptor 7D4 Protein SEQUENCE
  • SEQUENCE 312 AA; 34448 MW; FD97DD41B890B085 CRC64;

    MEAENLTELS KFLLLGLSDD PELQPVLFGL FLSMYLVTVL GNLLIILAVS

    SDSHLHTPMY FFLSNLSFVD ICFISTTVPK MLVSIQARSK DISYMGCLTQ

    VYFLMMFAGM DTFLLAVMAY DRFVAICHPL HYTVIMNPCL CGLLVLASWF

    IIFWFSLVHI LLMKRLTFST GTEIPHFFCE PAQVLKVACS NTLLNNIVLY

    VATALLGVFP VAGILFSYSQ IVSSLMGMSS TKGKYKAFST CGSHLCVVSL

    FYGTGLGVYL SSAVTHSSQS SSTASVMYAM VTPMLNPFIY SLRNKDVKGA

    LERLLSRADS CP

  • UCSC uc002mla.2; human.;



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