Human OLFM4 ELISA Kit

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  • Alternative name

    Human OLM4 ELISA Kit;Human antiapoptotic protein GW112 ELISA Kit;Human G-CSF-stimulated clone 1 protein ELISA Kit;Human hGC-1 ELISA Kit;Human hOLfD ELISA Kit;Human GW112 ELISA Kit;Human GC1 ELISA Kit;Human OlfD ELISA Kit;Human UNQ362 ELISA Kit;Human bA209J19.1 ELISA Kit;Human olfactomedin 4 ELISA Kit;Human olfactomedin-4 ELISA Kit;

  • Catalog
    E027272
  • species
    Human
  • GeneOLFM4
  • Standard CurveHuman OLFM4 ELISA Kit
  • Other Species Mouse Olfm4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of OLFM-4. No significant cross-reactivity or interference between OLFM-4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between OLFM-4 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman OLFM4 ELISA Kit allows for the in vitro quantitative determination of OLFM4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCancer
  • Product Description
    specifical
    Principle of the assay: OLFM-4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for OLFM-4. Standards or samples are then added to the microtiter plate wells and OLFM-4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of OLFM-4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for OLFM-4 are added to each well to "sandwich" the OLFM-4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain OLFM-4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OLFM-4 concentration in each sample is interpolated from this standard curve.
  • Human Olfactomedin-4 Protein information
  • Uniprot ID OLFM4_HUMAN
  • Uniprot AC Q6UX06; O95362; Q5VWG0; Q86T22;
  • UniGene Hs.508113;
  • GeneID 10562
  • KEGG hsa:10562;
  • Human Olfactomedin-4 Protein SEQUENCE
  • SEQUENCE 510 AA; 57280 MW; ACF03365D2164900 CRC64;

    MRPGLSFLLA LLFFLGQAAG DLGDVGPPIP SPGFSSFPGV DSSSSFSSSS

    RSGSSSSRSL GSGGSVSQLF SNFTGSVDDR GTCQCSVSLP DTTFPVDRVE

    RLEFTAHVLS QKFEKELSKV REYVQLISVY EKKLLNLTVR IDIMEKDTIS

    YTELDFELIK VEVKEMEKLV IQLKESFGGS SEIVDQLEVE IRNMTLLVEK

    LETLDKNNVL AIRREIVALK TKLKECEASK DQNTPVVHPP PTPGSCGHGG

    VVNISKPSVV QLNWRGFSYL YGAWGRDYSP QHPNKGLYWV APLNTDGRLL

    EYYRLYNTLD DLLLYINARE LRITYGQGSG TAVYNNNMYV NMYNTGNIAR

    VNLTTNTIAV TQTLPNAAYN NRFSYANVAW QDIDFAVDEN GLWVIYSTEA

    STGNMVISKL NDTTLQVLNT WYTKQYKPSA SNAFMVCGVL YATRTMNTRT

    EEIFYYYDTN TGKEGKLDIV MHKMQEKVQS INYNPFDQKL YVYNDGYLLN

    YDLSVLQKPQ

  • UCSC uc001vhl.4; human.;


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