Human OLFM4 ELISA Kit

Alternative name
Human OLM4 ELISA Kit;Human antiapoptotic protein GW112 ELISA Kit;Human G-CSF-stimulated clone 1 protein ELISA Kit;Human hGC-1 ELISA Kit;Human hOLfD ELISA Kit;Human GW112 ELISA Kit;Human GC1 ELISA Kit;Human OlfD ELISA Kit;Human UNQ362 ELISA Kit;Human bA209J19.1 ELISA Kit;Human olfactomedin 4 ELISA Kit;Human olfactomedin-4 ELISA Kit;
Catalog
E027272
species
Human
GeneOLFM4
SpecificityThis assay has high sensitivity and excellent specificity for detection of OLFM-4. No significant cross-reactivity or interference between OLFM-4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between OLFM-4 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman OLFM4 ELISA Kit allows for the in vitro quantitative determination of OLFM4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCancer
Product Description
specificalPrinciple of the assay: OLFM-4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for OLFM-4. Standards or samples are then added to the microtiter plate wells and OLFM-4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of OLFM-4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for OLFM-4 are added to each well to "sandwich" the OLFM-4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain OLFM-4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OLFM-4 concentration in each sample is interpolated from this standard curve.
Human Olfactomedin-4 Protein information
Uniprot ID OLFM4_HUMAN
Uniprot AC Q6UX06; O95362; Q5VWG0; Q86T22;
UniGene Hs.508113;
GeneID 10562
KEGG hsa:10562;
Human Olfactomedin-4 Protein SEQUENCE
SEQUENCE 510 AA; 57280 MW; ACF03365D2164900 CRC64;
MRPGLSFLLA LLFFLGQAAG DLGDVGPPIP SPGFSSFPGV DSSSSFSSSS
RSGSSSSRSL GSGGSVSQLF SNFTGSVDDR GTCQCSVSLP DTTFPVDRVE
RLEFTAHVLS QKFEKELSKV REYVQLISVY EKKLLNLTVR IDIMEKDTIS
YTELDFELIK VEVKEMEKLV IQLKESFGGS SEIVDQLEVE IRNMTLLVEK
LETLDKNNVL AIRREIVALK TKLKECEASK DQNTPVVHPP PTPGSCGHGG
VVNISKPSVV QLNWRGFSYL YGAWGRDYSP QHPNKGLYWV APLNTDGRLL
EYYRLYNTLD DLLLYINARE LRITYGQGSG TAVYNNNMYV NMYNTGNIAR
VNLTTNTIAV TQTLPNAAYN NRFSYANVAW QDIDFAVDEN GLWVIYSTEA
STGNMVISKL NDTTLQVLNT WYTKQYKPSA SNAFMVCGVL YATRTMNTRT
EEIFYYYDTN TGKEGKLDIV MHKMQEKVQS INYNPFDQKL YVYNDGYLLN
YDLSVLQKPQ
UCSC uc001vhl.4; human.;

Category

Human OLFM4 ELISA Kit