Human OCLN ELISA Kit

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  • Alternative name

    Human BLCPMG ELISA Kit;Human PPP1R115 ELISA Kit;Human occludin ELISA Kit;Human phosphatase 1, regulatory subunit 115 ELISA Kit;Human tight junction protein occludin ELISA Kit;

  • Catalog
    E027218
  • species
    Human
  • GeneOCLN
  • Other Species Mouse Ocln ELISA KitCanine OCLN ELISA KitChicken OCLN ELISA KitRat Ocln ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human OCLN. No significant cross-reactivity or interference between human OCLN and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human OCLN is typically less than 5.8 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
  • Intended UseHuman OCLN ELISA Kit allows for the in vitro quantitative determination of OCLN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for OCLN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any OCLN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OCLN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OCLN bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Occludin Protein information
  • Uniprot ID OCLN_HUMAN
  • Uniprot AC Q8N6K1;
  • UniGene Hs.592605;
  • GeneID 100506658
  • KEGG hsa:100506658;
  • Human Occludin Protein SEQUENCE
  • SEQUENCE 522 AA; 59144 MW; A0CF9574BCF6E974 CRC64;

    MSSRPLESPP PYRPDEFKPN HYAPSNDIYG GEMHVRPMLS QPAYSFYPED

    EILHFYKWTS PPGVIRILSM LIIVMCIAIF ACVASTLAWD RGYGTSLLGG

    SVGYPYGGSG FGSYGSGYGY GYGYGYGYGG YTDPRAAKGF MLAMAAFCFI

    AALVIFVTSV IRSEMSRTRR YYLSVIIVSA ILGIMVFIAT IVYIMGVNPT

    AQSSGSLYGS QIYALCNQFY TPAATGLYVD QYLYHYCVVD PQEAIAIVLG

    FMIIVAFALI IFFAVKTRRK MDRYDKSNIL WDKEHIYDEQ PPNVEEWVKN

    VSAGTQDVPS PPSDYVERVD SPMAYSSNGK VNDKRFYPES SYKSTPVPEV

    VQELPLTSPV DDFRQPRYSS GGNFETPSKR APAKGRAGRS KRTEQDHYET

    DYTTGGESCD ELEEDWIREY PPITSDQQRQ LYKRNFDTGL QEYKSLQSEL

    DEINKELSRL DKELDDYREE SEEYMAAADE YNRLKQVKGS ADYKSKKNHC

    KQLKSKLSHI KKMVGDYDRQ KT

  • UCSC uc003jwu.3; human. [Q16625-1];


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