Human OB ELISA Kit

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  • Alternative name

    Leptin ELISA KIT; Obese protein ELISA KIT; Obesity factorLEP ELISA KIT; OB ELISA KIT; OBS ELISA KIT

  • Catalog
    E027207
  • species
    Human
  • GeneOB
  • Standard CurveHuman OB ELISA Kit
  • Other Species Mouse OB-R ELISA KitMouse LR/Ob-R ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Obestatin (OB). No significant cross-reactivity or interference between Obestatin (OB) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 34pg/mL.
  • Intended UseHuman OB ELISA Kit allows for the in vitro quantitative determination of OB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Obestatin (OB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Obestatin (OB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Obestatin (OB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Obestatin (OB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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