Human OGT ELISA Kit

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  • Alternative name

    UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit ELISA KIT; O-GlcNAc transferase subunit p110 ELISA KIT; O-linked N-acetylglucosamine transferase 110 kDa subunit ELISA KIT; OGTOGT ELISA KIT; OGT ELISA KIT

  • Catalog
    E027193
  • species
    Human
  • GeneOGT
  • Other Species Mouse OGT ELISA KitRat Ogt ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human OGT. No significant cross-reactivity or interference between human OGT and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human OGT is typically less than 9.38 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
  • Intended UseHuman OGT ELISA Kit allows for the in vitro quantitative determination of OGT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for OGT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any OGT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OGT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OGT bound in the initial step. The color development is stopped and the intensity of the color is measured.


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