Human MGMP ELISA Kit

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  • Alternative name

    Methylated-DNA--protein-cysteine methyltransferase ELISA KIT; 6-O-methylguanine-DNA methyltransferase ELISA KIT; MGMT ELISA KIT; DNA repair MTase ELISA KIT; O-6-methylguanine-DNA-alkyltransferaseMGT1 ELISA KIT; YDL200C ELISA KIT; D1204 ELISA KIT; MGMT ELISA KIT

  • Catalog
    E027190
  • species
    Human
  • GeneMGMP
  • Standard CurveHuman MGMP ELISA Kit
  • Other Species Mouse MGMP ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman MGMP ELISA Kit allows for the in vitro quantitative determination of MGMP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Principle of the Assay: MGMP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MGMP antibody and an MGMP-HRP conjugate. The assay sample and buffer are incubated together with MGMP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MGMP concentration since MGMP from samples and MGMP-HRP conjugate compete for the anti-MGMP antibody binding site. Since the number of sites is limited, as more sites are occupied by MGMP from the sample, fewer sites are left to bind MGMP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MGMP concentration in each sample is interpolated from this standard curve.



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