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Human NUP50 ELISA Kit

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  • Alternative name

    Human 50 kDa nucleoporin ELISA Kit;Human Nuclear pore-associated protein 60 kDa-like ELISA Kit;Human nucleoporin Nup50 ELISA Kit;Human NPAP60L ELISA Kit;Human NPAP60 ELISA Kit;Human nucleoporin 50 ELISA Kit;Human nuclear pore complex protein Nup50 ELISA Kit;Human nuclear pore-associated protein 60L ELISA Kit;Human nucleoporin 50kD ELISA Kit;Human nucleoporin 50kDa ELISA Kit;
  • Catalog
    E027180
  • species
    Human
  • GeneNUP50
  • Other Species Mouse Nup50 ELISA KitRat Nup50 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human NUP50. No significant cross-reactivity or interference between Human NUP50 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human NUP50 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman NUP50 ELISA Kit allows for the in vitro quantitative determination of NUP50 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NUP50 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NUP50. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human NUP50 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NUP50, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human NUP50. You can calculate the concentration of Human NUP50 in the samples by comparing the OD of the samples to the standard curve.
  • Human Nuclear pore complex protein Nup50 Protein information
  • Uniprot ID NUP50_HUMAN
  • Uniprot AC Q9UKX7; B1AHA4; B2RB15; O75644; Q8N6V5; Q9NPM9; Q9NPR6; Q9P1K5;
  • UniGene Hs.475103;
  • GeneID 10762
  • KEGG hsa:10762;
  • Human Nuclear pore complex protein Nup50 Protein SEQUENCE

  • SEQUENCE 468 AA; 50144 MW; 1F2775AE9AC8FAC4 CRC64;

    MAKRNAEKEL TDRNWDQEDE AEEVGTFSMA SEEVLKNRAI KKAKRRNVGF

    ESDTGGAFKG FKGLVVPSGG GRFSGFGSGA GGKPLEGLSN GNNITSAPPF

    ASAKAAADPK VAFGSLAANG PTTLVDKVSN PKTNGDSQQP SSSGLASSKA

    CVGNAYHKQL AALNCSVRDW IVKHVNTNPL CDLTPIFKDY EKYLANIEQQ

    HGNSGRNSES ESNKVAAETQ SPSLFGSTKL QQESTFLFHG NKTEDTPDKK

    MEVASEKKTD PSSLGATSAS FNFGKKVDSS VLGSLSSVPL TGFSFSPGNS

    SLFGKDTTQS KPVSSPFPTK PLEGQAEGDS GECKGGDEEE NDEPPKVVVT

    EVKEEDAFYS KKCKLFYKKD NEFKEKGIGT LHLKPTANQK TQLLVRADTN

    LGNILLNVLI PPNMPCTRTG KNNVLIVCVP NPPIDEKNAT MPVTMLIRVK

    TSEDADELHK ILLEKKDA

  • UCSC uc003bfr.4; human. [Q9UKX7-1];


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