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Human NPM1 ELISA Kit

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  • Alternative name

    Human NPM ELISA Kit;Human Nucleolar phosphoprotein B23 ELISA Kit;Human nucleolar protein NO38 ELISA Kit;Human Numatrin ELISA Kit;Human B23 ELISA Kit;Human nucleophosmin ELISA Kit;Human nucleophosmin (nucleolar phosphoprotein B23, numatrin) ELISA Kit;Human nucleophosmin/nucleoplasmin family, member 1 ELISA Kit;Human testicular tissue protein Li 128 ELISA Kit;
  • Catalog
    E027075
  • species
    Human
  • GeneNPM1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human NPM1. No significant cross-reactivity or interference between human NPM1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human NPM1 is typically less than 5.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
  • Intended UseHuman NPM1 ELISA Kit allows for the in vitro quantitative determination of NPM1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NPM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NPM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NPM1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NPM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Nucleophosmin Protein information
  • Uniprot ID NPM_HUMAN
  • Uniprot AC Q8WTW5; Q96AT6; Q96DC4; Q96EA5; Q9BYG9; Q9UDJ7;
  • UniGene Hs.557550;
  • GeneID 4869
  • KEGG hsa:4869;
  • Human Nucleophosmin Protein SEQUENCE

  • SEQUENCE 294 AA; 32575 MW; 620BC7BA2E4A0054 CRC64;

    MEDSMDMDMS PLRPQNYLFG CELKADKDYH FKVDNDENEH QLSLRTVSLG

    AGAKDELHIV EAEAMNYEGS PIKVTLATLK MSVQPTVSLG GFEITPPVVL

    RLKCGSGPVH ISGQHLVAVE EDAESEDEEE EDVKLLSISG KRSAPGGGSK

    VPQKKVKLAA DEDDDDDDEE DDDEDDDDDD FDDEEAEEKA PVKKSIRDTP

    AKNAQKSNQN GKDSKPSSTP RSKGQESFKK QEKTPKTPKG PSSVEDIKAK

    MQASIEKGGS LPKVEAKFIN YVKNCFRMTD QEAIQDLWQW RKSL

  • UCSC uc003mbh.4; human. [P06748-1];



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