Human NCL ELISA Kit

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  • Alternative name

    Human Protein C23 ELISA Kit;Human C23 ELISA Kit;Human nucleolin ELISA Kit;

  • Catalog
    E027070
  • species
    Human
  • GeneNCL
  • Standard CurveHuman NCL ELISA Kit
  • Other Species Mouse Ncl ELISA KitChicken NCL ELISA KitMonkey NCL ELISA KitRat Ncl ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Nucleolin (NCL). No significant cross-reactivity or interference between Nucleolin (NCL) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.057ng/mL.
  • Intended UseHuman NCL ELISA Kit allows for the in vitro quantitative determination of NCL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nucleolin (NCL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nucleolin (NCL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nucleolin (NCL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nucleolin (NCL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Nucleolin Protein information
  • Uniprot ID NUCL_HUMAN
  • Uniprot AC P19338; Q53SK1; Q8NB06; Q9UCF0; Q9UDG1;
  • UniGene Hs.79110;
  • GeneID 4691
  • KEGG hsa:4691;
  • Human Nucleolin Protein SEQUENCE
  • SEQUENCE 710 AA; 76614 MW; C97F6E34E5CA6727 CRC64;

    MVKLAKAGKN QGDPKKMAPP PKEVEEDSED EEMSEDEEDD SSGEEVVIPQ

    KKGKKAAATS AKKVVVSPTK KVAVATPAKK AAVTPGKKAA ATPAKKTVTP

    AKAVTTPGKK GATPGKALVA TPGKKGAAIP AKGAKNGKNA KKEDSDEEED

    DDSEEDEEDD EDEDEDEDEI EPAAMKAAAA APASEDEDDE DDEDDEDDDD

    DEEDDSEEEA METTPAKGKK AAKVVPVKAK NVAEDEDEEE DDEDEDDDDD

    EDDEDDDDED DEEEEEEEEE EPVKEAPGKR KKEMAKQKAA PEAKKQKVEG

    TEPTTAFNLF VGNLNFNKSA PELKTGISDV FAKNDLAVVD VRIGMTRKFG

    YVDFESAEDL EKALELTGLK VFGNEIKLEK PKGKDSKKER DARTLLAKNL

    PYKVTQDELK EVFEDAAEIR LVSKDGKSKG IAYIEFKTEA DAEKTFEEKQ

    GTEIDGRSIS LYYTGEKGQN QDYRGGKNST WSGESKTLVL SNLSYSATEE

    TLQEVFEKAT FIKVPQNQNG KSKGYAFIEF ASFEDAKEAL NSCNKREIEG

    RAIRLELQGP RGSPNARSQP SKTLFVKGLS EDTTEETLKE SFDGSVRARI

    VTDRETGSSK GFGFVDFNSE EDAKAAKEAM EDGEIDGNKV TLDWAKPKGE

    GGFGGRGGGR GGFGGRGGGR GGRGGFGGRG RGGFGGRGGF RGGRGGGGDH

    KPQGKKTKFE

  • UCSC uc002vru.4; human.;


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