Human NUSAP1 ELISA Kit

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  • Alternative name

    Human NUSAP ELISA Kit;Human ANKT ELISA Kit;Human BM037 ELISA Kit;Human LNP ELISA Kit;Human PRO0310p1 ELISA Kit;Human Q0310 ELISA Kit;Human SAPL ELISA Kit;Human nucleolar and spindle associated protein 1 ELISA Kit;Human nucleolar and spindle-associated protein 1 ELISA Kit;Human nucleolar protein ANKT ELISA Kit;

  • Catalog
    E027043
  • species
    Human
  • GeneNUSAP1
  • Other Species Mouse Nusap1 ELISA KitBovine NUSAP1 ELISA KitChicken NUSAP1 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.094ng/ml
  • Intended UseHuman NUSAP1 ELISA Kit allows for the in vitro quantitative determination of NUSAP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-NUSAP1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-NUSAP1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the NUSAP1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of NUSAP1 can be calculated.
  • Human Nucleolar and spindle-associated protein 1 Protein information
  • Uniprot ID NUSAP_HUMAN
  • Uniprot AC Q9GZM9; Q9NZ85; Q9UI70;
  • UniGene Hs.615092;
  • GeneID 51203
  • KEGG hsa:51203;
  • Human Nucleolar and spindle-associated protein 1 Protein SEQUENCE
  • SEQUENCE 441 AA; 49452 MW; 1400E1B9F3FB727F CRC64;

    MIIPSLEELD SLKYSDLQNL AKSLGLRANL RATKLLKALK GYIKHEARKG

    NENQDESQTS ASSCDETEIQ ISNQEEAERQ PLGHVTKTRR RCKTVRVDPD

    SQQNHSEIKI SNPTEFQNHE KQESQDLRAT AKVPSPPDEH QEAENAVSSG

    NRDSKVPSEG KKSLYTDESS KPGKNKRTAI TTPNFKKLHE AHFKEMESID

    QYIERKKKHF EEHNSMNELK QQPINKGGVR TPVPPRGRLS VASTPISQRR

    SQGRSCGPAS QSTLGLKGSL KRSAISAAKT GVRFSAATKD NEHKRSLTKT

    PARKSAHVTV SGGTPKGEAV LGTHKLKTIT GNSAAVITPF KLTTEATQTP

    VSNKKPVFDL KASLSRPLNY EPHKGKLKPW GQSKENNYLN QHVNRINFYK

    KTYKQPHLQT KEEQRKKREQ ERKEKKAKVL GMRRGLILAE D

  • UCSC uc001znr.5; human. [Q9BXS6-1];


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