Human NFYC ELISA Kit

Alternative name
Human CAAT box DNA-binding protein subunit C ELISA Kit;Human nuclear transcription factor Y subunit C ELISA Kit;Human NF-YC ELISA Kit;Human transactivator HSM-1/2 ELISA Kit;Human CBF-C ELISA Kit;Human CBFC ELISA Kit;Human H1TF2A ELISA Kit;Human HAP5 ELISA Kit;Human HSM ELISA Kit;Human nuclear transcription factor Y subunit gamma ELISA Kit;Human CCAAT binding factor subunit C ELISA Kit;Human CCAAT transcription binding factor subunit gamma ELISA Kit;Human histone H1 transcription factor large subunit 2A ELISA Kit;Human nuclear transcription factor Y, gamma ELISA Kit;Human transactivator HSM-1 ELISA Kit;Human transcription factor NF-Y, C subunit ELISA Kit;
Catalog
E027025
species
Human
GeneNFYC
SpecificityThis assay has high sensitivity and excellent specificity for detection of Nuclear Transcription Factor Y Gamma (NFYC). No significant cross-reactivity or interference between Nuclear Transcription Factor Y Gamma (NFYC) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.114ng/mL.
Intended UseHuman NFYC ELISA Kit allows for the in vitro quantitative determination of NFYC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Transcription Factor Y Gamma (NFYC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Transcription Factor Y Gamma (NFYC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Transcription Factor Y Gamma (NFYC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nuclear Transcription Factor Y Gamma (NFYC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Nuclear transcription factor Y subunit gamma Protein information
Uniprot ID NFYC_HUMAN
Uniprot AC Q5T6L1; Q5TZR6; Q92869; Q9HBX1; Q9NXB5; Q9UM67; Q9UML0; Q9UMT7;
UniGene Hs.713051;
GeneID 4802
KEGG hsa:4802;
Human Nuclear transcription factor Y subunit gamma Protein SEQUENCE
SEQUENCE 458 AA; 50302 MW; B17B2C7F622653B9 CRC64;
MSTEGGFGGT SSSDAQQSLQ SFWPRVMEEI RNLTVKDFRV QELPLARIKK
IMKLDEDVKM ISAEAPVLFA KAAQIFITEL TLRAWIHTED NKRRTLQRND
IAMAITKFDQ FDFLIDIVPR DELKPPKRQE EVRQSVTPAE PVQYYFTLAQ
QPTAVQVQGQ QQGQQTTSST TTIQPGQIII AQPQQGQTTP VTMQVGEGQQ
VQIVQAQPQG QAQQAQSGTG QTMQVMQQII TNTGEIQQIP VQLNAGQLQY
IRLAQPVSGT QVVQGQIQTL ATNAQQGQRN ASQGKPRRCL KETLQITQTE
VQQGQQQFSQ FTDGQRNSVQ QARVSELTGE AEPREVKATG NSTPCTSSLP
TTHPPSHRAG ASCVCCSQPQ QSSTSPPPSD ALQWVVVEVS GTPNQLETHR
ELHAPLPGMT SLSPLHPSQQ LYQIQQVTMP AGQDLAQPMF IQSANQPSDG
QAPQVTGD
UCSC uc001cfx.6; human. [Q13952-1];

Category

Human NFYC ELISA Kit