Human ADAM33 ELISA Kit

Alternative name
Human ADAM 33 ELISA Kit;Human C20orf153 ELISA Kit;Human DJ964F7.1 ELISA Kit;Human ADAM metallopeptidase domain 33 ELISA Kit;Human disintegrin and metalloproteinase domain-containing protein 33 ELISA Kit;Human a disintegrin and metalloprotease 33 ELISA Kit;Human a disintegrin and metalloproteinase domain 33 ELISA Kit;Human disintegrin and reprolysin metalloproteinase family protein ELISA Kit;
Catalog
E000027
species
Human
GeneADAM33
Standard Curve
Other Species Mouse Adam33 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloprotease 33 (ADAM33). No significant cross-reactivity or interference between A Disintegrin And Metalloprotease 33 (ADAM33) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.054ng/mL.
Intended UseHuman ADAM33 ELISA Kit allows for the in vitro quantitative determination of ADAM33 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 33 (ADAM33). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 33 (ADAM33). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloprotease 33 (ADAM33), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of A Disintegrin And Metalloprotease 33 (ADAM33) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Disintegrin and metalloproteinase domain-containing protein 33 Protein information
Uniprot ID ADA33_HUMAN
Uniprot AC Q9BZ11; A0A1K6; Q5JT75; Q5JT76; Q8N0W6;
UniGene Hs.173716;
GeneID 80332
KEGG hsa:80332;
Human Disintegrin and metalloproteinase domain-containing protein 33 Protein SEQUENCE
SEQUENCE 813 AA; 87739 MW; 90713A99668D5569 CRC64;
MGWRPRRARG TPLLLLLLLL LLWPVPGAGV LQGHIPGQPV TPHWVLDGQP
WRTVSLEEPV SKPDMGLVAL EAEGQELLLE LEKNHRLLAP GYIETHYGPD
GQPVVLAPNH TDHCHYQGRV RGFPDSWVVL CTCSGMSGLI TLSRNASYYL
RPWPPRGSKD FSTHEIFRME QLLTWKGTCG HRDPGNKAGM TSLPGGPQSR
GRREARRTRK YLELYIVADH TLFLTRHRNL NHTKQRLLEV ANYVDQLLRT
LDIQVALTGL EVWTERDRSR VTQDANATLW AFLQWRRGLW AQRPHDSAQL
LTGRAFQGAT VGLAPVEGMC RAESSGGVST DHSELPIGAA ATMAHEIGHS
LGLSHDPDGC CVEAAAESGG CVMAAATGHP FPRVFSACSR RQLRAFFRKG
GGACLSNAPD PGLPVPPALC GNGFVEAGEE CDCGPGQECR DLCCFAHNCS
LRPGAQCAHG DCCVRCLLKP AGALCRQAMG DCDLPEFCTG TSSHCPPDVY
LLDGSPCARG SGYCWDGACP TLEQQCQQLW GPGSHPAPEA CFQVVNSAGD
AHGNCGQDSE GHFLPCAGRD ALCGKLQCQG GKPSLLAPHM VPVDSTVHLD
GQEVTCRGAL ALPSAQLDLL GLGLVEPGTQ CGPRMVCQSR RCRKNAFQEL
QRCLTACHSH GVCNSNHNCH CAPGWAPPFC DKPGFGGSMD SGPVQAENHD
TFLLAMLLSV LLPLLPGAGL AWCCYRLPGA HLQRCSWGCR RDPACSGPKD
GPHRDHPLGG VHPMELGPTA TGQPWPLDPE NSHEPSSHPE KPLPAVSPDP
QADQVQMPRS CLW
UCSC uc002wit.5; human. [Q9BZ11-1];

Category

Human ADAM33 ELISA Kit