Human NR1I2 ELISA Kit

Alternative name
Human orphan nuclear receptor PAR1 ELISA Kit;Human orphan nuclear receptor PXR ELISA Kit;Human pregnane X receptor ELISA Kit;Human steroid and xenobiotic receptor ELISA Kit;Human SXR ELISA Kit;Human PXR ELISA Kit;Human BXR ELISA Kit;Human ONR1 ELISA Kit;Human PAR ELISA Kit;Human PAR1 ELISA Kit;Human PAR2 ELISA Kit;Human PARq ELISA Kit;Human PRR ELISA Kit;Human SAR ELISA Kit;Human nuclear receptor subfamily 1 group I member 2 ELISA Kit;Human pregnane X nuclear receptor variant 2 ELISA Kit;
Catalog
E026989
species
Human
GeneNR1I2
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseHuman NR1I2 ELISA Kit allows for the in vitro quantitative determination of NR1I2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-PXR antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-PXR antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PXR amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PXR can be calculated.
Human Nuclear receptor subfamily 1 group I member 2 Protein information
Uniprot ID NR1I2_HUMAN
Uniprot AC Q9UJ26; Q9UJ27; Q9UNW4;
UniGene Hs.7303;
GeneID 8856
KEGG hsa:8856;
Human Nuclear receptor subfamily 1 group I member 2 Protein SEQUENCE
SEQUENCE 434 AA; 49762 MW; 1DF6A2AE3109C4DA CRC64;
MEVRPKESWN HADFVHCEDT ESVPGKPSVN ADEEVGGPQI CRVCGDKATG
YHFNVMTCEG CKGFFRRAMK RNARLRCPFR KGACEITRKT RRQCQACRLR
KCLESGMKKE MIMSDEAVEE RRALIKRKKS ERTGTQPLGV QGLTEEQRMM
IRELMDAQMK TFDTTFSHFK NFRLPGVLSS GCELPESLQA PSREEAAKWS
QVRKDLCSLK VSLQLRGEDG SVWNYKPPAD SGGKEIFSLL PHMADMSTYM
FKGIISFAKV ISYFRDLPIE DQISLLKGAA FELCQLRFNT VFNAETGTWE
CGRLSYCLED TAGGFQQLLL EPMLKFHYML KKLQLHEEEY VLMQAISLFS
PDRPGVLQHR VVDQLQEQFA ITLKSYIECN RPQPAHRFLF LKIMAMLTEL
RSINAQHTQR LLRIQDIHPF ATPLMQELFG ITGS
UCSC uc003edk.3; human. [O75469-1];

Category

Human NR1I2 ELISA Kit