Human NRIP1 ELISA Kit

Alternative name
Human nuclear factor RIP140 ELISA Kit;Human receptor-interacting protein 140 ELISA Kit;Human RIP140 ELISA Kit;Human nuclear receptor interacting protein 1 ELISA Kit;Human nuclear receptor-interacting protein 1 ELISA Kit;
Catalog
E026978
species
Human
GeneNRIP1
Standard Curve
Other Species Mouse Nrip1 ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 nmol/L.
Intended UseHuman NRIP1 ELISA Kit allows for the in vitro quantitative determination of NRIP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalFor samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This NTx ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NTx. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY NTx ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NTx antibody and an NTx-HRP conjugate. The assay sample and buffer are incubated together with NTx-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NTx concentration since NTx from samples and NTx-HRP conjugate compete for the anti-NTx antibody binding site. Since the number of sites is limited, as more sites are occupied by NTx from the sample, fewer sites are left to bind NTx-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NTx concentration in each sample is interpolated from this standard curve.
Human Nuclear receptor-interacting protein 1 Protein information
Uniprot ID NRIP1_HUMAN
Uniprot AC P48552; Q8IWE8;
UniGene Hs.155017;
GeneID 8204
KEGG hsa:8204;
Human Nuclear receptor-interacting protein 1 Protein SEQUENCE
SEQUENCE 1158 AA; 126942 MW; 81FC424968E9A5F6 CRC64;
MTHGEELGSD VHQDSIVLTY LEGLLMHQAA GGSGTAVDKK SAGHNEEDQN
FNISGSAFPT CQSNGPVLNT HTYQGSGMLH LKKARLLQSS EDWNAAKRKR
LSDSIMNLNV KKEALLAGMV DSVPKGKQDS TLLASLLQSF SSRLQTVALS
QQIRQSLKEQ GYALSHDSLK VEKDLRCYGV ASSHLKTLLK KSKVKDQKPD
TNLPDVTKNL IRDRFAESPH HVGQSGTKVM SEPLSCAARL QAVASMVEKR
ASPATSPKPS VACSQLALLL SSEAHLQQYS REHALKTQNA NQAASERLAA
MARLQENGQK DVGSYQLPKG MSSHLNGQAR TSSSKLMASK SSATVFQNPM
GIIPSSPKNA GYKNSLERNN IKQAANNSLL LHLLKSQTIP KPMNGHSHSE
RGSIFEESST PTTIDEYSDN NPSFTDDSSG DESSYSNCVP IDLSCKHRTE
KSESDQPVSL DNFTQSLLNT WDPKVPDVDI KEDQDTSKNS KLNSHQKVTL
LQLLLGHKNE ENVEKNTSPQ GVHNDVSKFN TQNYARTSVI ESPSTNRTTP
VSTPPLLTSS KAGSPINLSQ HSLVIKWNSP PYVCSTQSEK LTNTASNHSM
DLTKSKDPPG EKPAQNEGAQ NSATFSASKL LQNLAQCGMQ SSMSVEEQRP
SKQLLTGNTD KPIGMIDRLN SPLLSNKTNA VEENKAFSSQ PTGPEPGLSG
SEIENLLERR TVLQLLLGNP NKGKSEKKEK TPLRDESTQE HSERALSEQI
LMVKIKSEPC DDLQIPNTNV HLSHDAKSAP FLGMAPAVQR SAPALPVSED
FKSEPVSPQD FSFSKNGLLS RLLRQNQDSY LADDSDRSHR NNEMALLESK
NLCMVPKKRK LYTEPLENPF KKMKNNIVDA ANNHSAPEVL YGSLLNQEEL
KFSRNDLEFK YPAGHGSASE SEHRSWARES KSFNVLKQLL LSENCVRDLS
PHRSNSVADS KKKGHKNNVT NSKPEFSISS LNGLMYSSTQ PSSCMDNRTF
SYPGVVKTPV SPTFPEHLGC AGSRPESGLL NGCSMPSEKG PIKWVITDAE
KNEYEKDSPR LTKTNPILYY MLQKGGNSVT SRETQDKDIW REASSAESVS
QVTAKEELLP TAETKASFFN LRSPYNSHMG NNASRPHSAN GEVYGLLGSV
LTIKKESE
UCSC uc002yjx.2; human.;

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Human NRIP1 ELISA Kit

Human nuclear factor RIP140 ELISA Kit;Human receptor-interacting protein 140 ELISA Kit;Human RIP140 ELISA Kit;Human nuclear receptor interacting protein 1 ELISA Kit;Human nuclear receptor-interacting protein 1 ELISA Kit;