Human NFIL3 ELISA Kit

Alternative name
Human E4 promoter-binding protein 4 ELISA Kit;Human interleukin-3 promoter transcriptional activator ELISA Kit;Human interleukin-3-binding protein 1 ELISA Kit;Human transcriptional activator NF-IL3A ELISA Kit;Human E4BP4 ELISA Kit;Human IL3BP1 ELISA Kit;Human NF-IL3A ELISA Kit;Human NFIL3A ELISA Kit;Human nuclear factor, interleukin 3 regulated ELISA Kit;Human nuclear factor interleukin-3-regulated protein ELISA Kit;
Catalog
E026926
species
Human
GeneNFIL3
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman NFIL3 ELISA Kit allows for the in vitro quantitative determination of NFIL3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalPrinciple of the Assay: NFIL3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NFIL3 antibody and an NFIL3-HRP conjugate. The assay sample and buffer are incubated together with NFIL3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NFIL3 concentration since NFIL3 from samples and NFIL3-HRP conjugate compete for the anti-NFIL3 antibody binding site. Since the number of sites is limited, as more sites are occupied by NFIL3 from the sample, fewer sites are left to bind NFIL3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NFIL3 concentration in each sample is interpolated from this standard curve.
Human Nuclear factor interleukin-3-regulated protein Protein information
Uniprot ID NFIL3_HUMAN
Uniprot AC Q16649; B2R9Y8; Q14211; Q6FGQ8; Q96HS0;
UniGene Hs.79334;
GeneID 4783
KEGG hsa:4783;
Human Nuclear factor interleukin-3-regulated protein Protein SEQUENCE
SEQUENCE 462 AA; 51472 MW; D19946AAC774C3E7 CRC64;
MQLRKMQTVK KEQASLDASS NVDKMMVLNS ALTEVSEDST TGEELLLSEG
SVGKNKSSAC RRKREFIPDE KKDAMYWEKR RKNNEAAKRS REKRRLNDLV
LENKLIALGE ENATLKAELL SLKLKFGLIS STAYAQEIQK LSNSTAVYFQ
DYQTSKSNVS SFVDEHEPSM VSSSCISVIK HSPQSSLSDV SEVSSVEHTQ
ESSVQGSCRS PENKFQIIKQ EPMELESYTR EPRDDRGSYT ASIYQNYMGN
SFSGYSHSPP LLQVNRSSSN SPRTSETDDG VVGKSSDGED EQQVPKGPIH
SPVELKHVHA TVVKVPEVNS SALPHKLRIK AKAMQIKVEA FDNEFEATQK
LSSPIDMTSK RHFELEKHSA PSMVHSSLTP FSVQVTNIQD WSLKSEHWHQ
KELSGKTQNS FKTGVVEMKD SGYKVSDPEN LYLKQGIANL SAEVVSLKRL
IATQPISASD SG
UCSC uc004arh.3; human.;

Category

Human NFIL3 ELISA Kit