Human NFKB1 ELISA Kit

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  • Alternative name

    Human DNA-binding factor KBF1 ELISA Kit;Human EBP-1 ELISA Kit;Human nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 ELISA Kit;Human CVID12 ELISA Kit;Human KBF1 ELISA Kit;Human NF-kB1 ELISA Kit;Human NF-kappa-B ELISA Kit;Human NF-kappaB ELISA Kit;Human NFKB-p105 ELISA Kit;Human NFKB-p50 ELISA Kit;Human NFkappaB ELISA Kit;Human p105 ELISA Kit;Human p50 ELISA Kit;Human nuclear factor kappa B subunit 1 ELISA Kit;Human nuclear factor NF-kappa-B p105 subunit ELISA Kit;Human NF-kappabeta ELISA Kit;Human nuclear factor NF-kappa-B p50 subunit ELISA Kit;Human nuclear factor kappa-B DNA binding subunit ELISA Kit;

  • Catalog
    E026914
  • species
    Human
  • GeneNFKB1
  • Other Species Human NFKB1/p105 ELISA KitMouse Nfkb1 ELISA KitMouse NFKB1/p105 ELISA KitCanine NFKB1 ELISA KitChicken NFKB1 ELISA KitRat Nfkb1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human NF-kB. No significant cross-reactivity or interference between human NF-kB and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human NF-kB is typically less than 0.078 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
  • Intended UseHuman NFKB1 ELISA Kit allows for the in vitro quantitative determination of NFKB1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NF-kappaB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NF-kappaB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NF-kappaB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NF-kappaB bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Nuclear factor NF-kappa-B p50 subunit Protein information
  • Uniprot ID NFKB1_HUMAN
  • Uniprot AC P19838; A8K5Y5; B3KVE8; Q68D84; Q86V43; Q8N4X7; Q9NZC0;
  • UniGene Hs.618430;
  • GeneID 4790
  • KEGG hsa:4790;
  • Human Nuclear factor NF-kappa-B p50 subunit Protein SEQUENCE
  • SEQUENCE 968 AA; 105356 MW; 2A7C8FF86A10D1A8 CRC64;

    MAEDDPYLGR PEQMFHLDPS LTHTIFNPEV FQPQMALPTD GPYLQILEQP

    KQRGFRFRYV CEGPSHGGLP GASSEKNKKS YPQVKICNYV GPAKVIVQLV

    TNGKNIHLHA HSLVGKHCED GICTVTAGPK DMVVGFANLG ILHVTKKKVF

    ETLEARMTEA CIRGYNPGLL VHPDLAYLQA EGGGDRQLGD REKELIRQAA

    LQQTKEMDLS VVRLMFTAFL PDSTGSFTRR LEPVVSDAIY DSKAPNASNL

    KIVRMDRTAG CVTGGEEIYL LCDKVQKDDI QIRFYEEEEN GGVWEGFGDF

    SPTDVHRQFA IVFKTPKYKD INITKPASVF VQLRRKSDLE TSEPKPFLYY

    PEIKDKEEVQ RKRQKLMPNF SDSFGGGSGA GAGGGGMFGS GGGGGGTGST

    GPGYSFPHYG FPTYGGITFH PGTTKSNAGM KHGTMDTESK KDPEGCDKSD

    DKNTVNLFGK VIETTEQDQE PSEATVGNGE VTLTYATGTK EESAGVQDNL

    FLEKAMQLAK RHANALFDYA VTGDVKMLLA VQRHLTAVQD ENGDSVLHLA

    IIHLHSQLVR DLLEVTSGLI SDDIINMRND LYQTPLHLAV ITKQEDVVED

    LLRAGADLSL LDRLGNSVLH LAAKEGHDKV LSILLKHKKA ALLLDHPNGD

    GLNAIHLAMM SNSLPCLLLL VAAGADVNAQ EQKSGRTALH LAVEHDNISL

    AGCLLLEGDA HVDSTTYDGT TPLHIAAGRG STRLAALLKA AGADPLVENF

    EPLYDLDDSW ENAGEDEGVV PGTTPLDMAT SWQVFDILNG KPYEPEFTSD

    DLLAQGDMKQ LAEDVKLQLY KLLEIPDPDK NWATLAQKLG LGILNNAFRL

    SPAPSKTLMD NYEVSGGTVR ELVEALRQMG YTEAIEVIQA ASSPVKTTSQ

    AHSLPLSPAS TRQQIDELRD SDSVCDSGVE TSFRKLSFTE SLTSGASLLT

    LNKMPHDYGQ EGPLEGKI

  • UCSC uc011cep.2; human. [P19838-1];



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