Human NFKB1 ELISA Kit

Alternative name
Human DNA-binding factor KBF1 ELISA Kit;Human EBP-1 ELISA Kit;Human nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 ELISA Kit;Human CVID12 ELISA Kit;Human KBF1 ELISA Kit;Human NF-kB1 ELISA Kit;Human NF-kappa-B ELISA Kit;Human NF-kappaB ELISA Kit;Human NFKB-p105 ELISA Kit;Human NFKB-p50 ELISA Kit;Human NFkappaB ELISA Kit;Human p105 ELISA Kit;Human p50 ELISA Kit;Human nuclear factor kappa B subunit 1 ELISA Kit;Human nuclear factor NF-kappa-B p105 subunit ELISA Kit;Human NF-kappabeta ELISA Kit;Human nuclear factor NF-kappa-B p50 subunit ELISA Kit;Human nuclear factor kappa-B DNA binding subunit ELISA Kit;
Catalog
E026914
species
Human
GeneNFKB1
SpecificityThis assay has high sensitivity and excellent specificity for detection of human NF-kB. No significant cross-reactivity or interference between human NF-kB and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human NF-kB is typically less than 0.078 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman NFKB1 ELISA Kit allows for the in vitro quantitative determination of NFKB1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NF-kappaB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NF-kappaB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NF-kappaB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NF-kappaB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Nuclear factor NF-kappa-B p50 subunit Protein information
Uniprot ID NFKB1_HUMAN
Uniprot AC P19838; A8K5Y5; B3KVE8; Q68D84; Q86V43; Q8N4X7; Q9NZC0;
UniGene Hs.618430;
GeneID 4790
KEGG hsa:4790;
Human Nuclear factor NF-kappa-B p50 subunit Protein SEQUENCE
SEQUENCE 968 AA; 105356 MW; 2A7C8FF86A10D1A8 CRC64;
MAEDDPYLGR PEQMFHLDPS LTHTIFNPEV FQPQMALPTD GPYLQILEQP
KQRGFRFRYV CEGPSHGGLP GASSEKNKKS YPQVKICNYV GPAKVIVQLV
TNGKNIHLHA HSLVGKHCED GICTVTAGPK DMVVGFANLG ILHVTKKKVF
ETLEARMTEA CIRGYNPGLL VHPDLAYLQA EGGGDRQLGD REKELIRQAA
LQQTKEMDLS VVRLMFTAFL PDSTGSFTRR LEPVVSDAIY DSKAPNASNL
KIVRMDRTAG CVTGGEEIYL LCDKVQKDDI QIRFYEEEEN GGVWEGFGDF
SPTDVHRQFA IVFKTPKYKD INITKPASVF VQLRRKSDLE TSEPKPFLYY
PEIKDKEEVQ RKRQKLMPNF SDSFGGGSGA GAGGGGMFGS GGGGGGTGST
GPGYSFPHYG FPTYGGITFH PGTTKSNAGM KHGTMDTESK KDPEGCDKSD
DKNTVNLFGK VIETTEQDQE PSEATVGNGE VTLTYATGTK EESAGVQDNL
FLEKAMQLAK RHANALFDYA VTGDVKMLLA VQRHLTAVQD ENGDSVLHLA
IIHLHSQLVR DLLEVTSGLI SDDIINMRND LYQTPLHLAV ITKQEDVVED
LLRAGADLSL LDRLGNSVLH LAAKEGHDKV LSILLKHKKA ALLLDHPNGD
GLNAIHLAMM SNSLPCLLLL VAAGADVNAQ EQKSGRTALH LAVEHDNISL
AGCLLLEGDA HVDSTTYDGT TPLHIAAGRG STRLAALLKA AGADPLVENF
EPLYDLDDSW ENAGEDEGVV PGTTPLDMAT SWQVFDILNG KPYEPEFTSD
DLLAQGDMKQ LAEDVKLQLY KLLEIPDPDK NWATLAQKLG LGILNNAFRL
SPAPSKTLMD NYEVSGGTVR ELVEALRQMG YTEAIEVIQA ASSPVKTTSQ
AHSLPLSPAS TRQQIDELRD SDSVCDSGVE TSFRKLSFTE SLTSGASLLT
LNKMPHDYGQ EGPLEGKI
UCSC uc011cep.2; human. [P19838-1];

Category

Human NFKB1 ELISA Kit