Nuclear factor NF-kappa-B p105 subunit ELISA KIT; DNA-binding factor KBF1 ELISA KIT; EBP-1 ELISA KIT; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1Cleaved into the following chain:Nuclear factor NF-kappa-B p50 subunitNFKB1 ELISA KIT;
SpecificityThis assay has high sensitivity and excellent specificity for detection of NFKB1/p105. No significant cross-reactivity or interference between NFKB1/p105 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NFKB1/p105 and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman NFKB1/p105 ELISA Kit allows for the in vitro quantitative determination of NFKB1/p105 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the assay: NFKB1/p105 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NFKB1/p105 antibody and an NFKB1/p105-HRP conjugate. The assay sample and buffer are incubated together with NFKB1/p105-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NFKB1/p105 concentration since NFKB1/p105 from samples and NFKB1/p105-HRP conjugate compete for the anti-NFKB1/p105 antibody binding site. Since the number of sites is limited, as more sites are occupied by NFKB1/p105 from the sample, fewer sites are left to bind NFKB1/p105-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NFKB1/p105 concentration in each sample is interpolated from this standard curve.