Human NFIB ELISA Kit

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  • Alternative name

    Human NF1-B ELISA Kit;Human nuclear factor 1/B ELISA Kit;Human CCAAT-box-binding transcription factor ELISA Kit;Human CTF ELISA Kit;Human Nuclear factor I/B ELISA Kit;Human NF-I/B ELISA Kit;Human NFI-B ELISA Kit;Human TGGCA-binding protein ELISA Kit;Human HMGIC/NFIB ELISA Kit;Human NFI-RED ELISA Kit;Human NFIB2 ELISA Kit;Human NFIB3 ELISA Kit;Human nuclear factor I B ELISA Kit;Human nuclear factor 1 B-type ELISA Kit;

  • Catalog
    E026883
  • species
    Human
  • GeneNFIB
  • Standard CurveHuman NFIB ELISA Kit
  • Other Species Mouse Nfib ELISA KitBovine NFIB ELISA KitChicken NFIB ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of NFIB. No significant cross-reactivity or interference between NFIB and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human NFIB is typically less than 0.054ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addi
  • Intended UseHuman NFIB ELISA Kit allows for the in vitro quantitative determination of NFIB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NFIB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NFIB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NFIB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NFIB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Nuclear factor 1 B-type Protein information
  • Uniprot ID NFIB_HUMAN
  • Uniprot AC Q96J45;
  • UniGene Hs.644095;
  • GeneID 4781
  • KEGG hsa:4781;
  • Human Nuclear factor 1 B-type Protein SEQUENCE
  • SEQUENCE 420 AA; 47442 MW; 0F71635FE9D64FA4 CRC64;

    MMYSPICLTQ DEFHPFIEAL LPHVRAIAYT WFNLQARKRK YFKKHEKRMS

    KDEERAVKDE LLSEKPEIKQ KWASRLLAKL RKDIRQEYRE DFVLTVTGKK

    HPCCVLSNPD QKGKIRRIDC LRQADKVWRL DLVMVILFKG IPLESTDGER

    LMKSPHCTNP ALCVQPHHIT VSVKELDLFL AYYVQEQDSG QSGSPSHNDP

    AKNPPGYLED SFVKSGVFNV SELVRVSRTP ITQGTGVNFP IGEIPSQPYY

    HDMNSGVNLQ RSLSSPPSSK RPKTISIDEN MEPSPTGDFY PSPSSPAAGS

    RTWHERDQDM SSPTTMKKPE KPLFSSASPQ DSSPRLSTFP QHHHPGIPGV

    AHSVISTRTP PPPSPLPFPT QAILPPAPSS YFSHPTIRYP PHLNPQDTLK

    NYVPSYDPSS PQTSQSWYLG

  • UCSC uc003zld.4; human. [O00712-1];


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