Human NRCAM ELISA Kit

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  • Alternative name

    Human Nr-CAM ELISA Kit;Human neuronal surface protein Bravo ELISA Kit;Human hBravo ELISA Kit;Human NgCAM-related cell adhesion molecule ELISA Kit;Human Ng-CAM-related ELISA Kit;Human KIAA0343 ELISA Kit;Human neuronal cell adhesion molecule ELISA Kit;

  • Catalog
    E026823
  • species
    Human
  • GeneNRCAM
  • Standard CurveHuman NRCAM ELISA Kit
  • Other Species Mouse Nrcam ELISA KitRat Nrcam ELISA Kit
  • SpecificityNatural and recombinant human NRCAM
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Sample For quantitative detection of human NRCAM in cell culture supernates, serum and plas
  • Intended UseHuman NRCAM ELISA Kit allows for the in vitro quantitative determination of NRCAM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: Human NRCAM ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for NRCAM has been precoated onto 96-well plates. Standards (NSO, L30-N600) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NRCAM is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human NRCAM amount of sample captured in plate. Background: Neuronal cell adhesion molecule is a protein that in humans is encoded by the NRCAM gene. Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. Also, this gene is expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms.
  • Human Neuronal cell adhesion molecule Protein information
  • Uniprot ID NRCAM_HUMAN
  • Uniprot AC Q92823; A4D0S3; E9PDA4; O15051; O15179; Q14BM2; Q9UHI3; Q9UHI4;
  • UniGene Hs.21422;
  • GeneID 4897
  • KEGG hsa:4897;
  • Human Neuronal cell adhesion molecule Protein SEQUENCE
  • SEQUENCE 1304 AA; 143890 MW; 1F2698BC7DB5C363 CRC64;

    MQLKIMPKKK RLSAGRVPLI LFLCQMISAL EVPLDPKLLE DLVQPPTITQ

    QSPKDYIIDP RENIVIQCEA KGKPPPSFSW TRNGTHFDID KDPLVTMKPG

    TGTLIINIMS EGKAETYEGV YQCTARNERG AAVSNNIVVR PSRSPLWTKE

    KLEPITLQSG QSLVLPCRPP IGLPPPIIFW MDNSFQRLPQ SERVSQGLNG

    DLYFSNVLPE DTREDYICYA RFNHTQTIQQ KQPISVKVIS VDELNDTIAA

    NLSDTEFYGA KSSRERPPTF LTPEGNASNK EELRGNVLSL ECIAEGLPTP

    IIYWAKEDGM LPKNRTVYKN FEKTLQIIHV SEADSGNYQC IAKNALGAIH

    HTISVRVKAA PYWITAPQNL VLSPGEDGTL ICRANGNPKP RISWLTNGVP

    IEIAPDDPSR KIDGDTIIFS NVQERSSAVY QCNASNEYGY LLANAFVNVL

    AEPPRILTPA NTLYQVIANR PALLDCAFFG SPLPTIEWFK GAKGSALHED

    IYVLHENGTL EIPVAQKDST GTYTCVARNK LGMAKNEVHL EIKDPTWIVK

    QPEYAVVQRG SMVSFECKVK HDHTLSLTVL WLKDNRELPS DERFTVDKDH

    LVVADVSDDD SGTYTCVANT TLDSVSASAV LSVVAPTPTP APVYDVPNPP

    FDLELTDQLD KSVQLSWTPG DDNNSPITKF IIEYEDAMHK PGLWHHQTEV

    SGTQTTAQLK LSPYVNYSFR VMAVNSIGKS LPSEASEQYL TKASEPDKNP

    TAVEGLGSEP DNLVITWKPL NGFESNGPGL QYKVSWRQKD GDDEWTSVVV

    ANVSKYIVSG TPTFVPYLIK VQALNDMGFA PEPAVVMGHS GEDLPMVAPG

    NVRVNVVNST LAEVHWDPVP LKSIRGHLQG YRIYYWKTQS SSKRNRRHIE

    KKILTFQGSK THGMLPGLEP FSHYTLNVRV VNGKGEGPAS PDRVFNTPEG

    VPSAPSSLKI VNPTLDSLTL EWDPPSHPNG ILTEYTLKYQ PINSTHELGP

    LVDLKIPANK TRWTLKNLNF STRYKFYFYA QTSAGSGSQI TEEAVTTVDE

    AGILPPDVGA GKVQAVNPRI SNLTAAAAET YANISWEYEG PEHVNFYVEY

    GVAGSKEEWR KEIVNGSRSF FGLKGLMPGT AYKVRVGAVG DSGFVSSEDV

    FETGPAMASR QVDIATQGWF IGLMCAVALL ILILLIVCFI RRNKGGKYPV

    KEKEDAHADP EIQPMKEDDG TFGEYSDAED HKPLKKGSRT PSDRTVKKED

    SDDSLVDYGE GVNGQFNEDG SFIGQYSGKK EKEPAEGNES SEAPSPVNAM

    NSFV

  • UCSC uc003vfc.4; human. [Q92823-1];


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