Human NRCAM ELISA Kit

Alternative name
Human Nr-CAM ELISA Kit;Human neuronal surface protein Bravo ELISA Kit;Human hBravo ELISA Kit;Human NgCAM-related cell adhesion molecule ELISA Kit;Human Ng-CAM-related ELISA Kit;Human KIAA0343 ELISA Kit;Human neuronal cell adhesion molecule ELISA Kit;
Catalog
E026823
species
Human
GeneNRCAM
SpecificityNatural and recombinant human NRCAM
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity Sample For quantitative detection of human NRCAM in cell culture supernates, serum and plas
Intended UseHuman NRCAM ELISA Kit allows for the in vitro quantitative determination of NRCAM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: Human NRCAM ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for NRCAM has been precoated onto 96-well plates. Standards (NSO, L30-N600) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NRCAM is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human NRCAM amount of sample captured in plate. Background: Neuronal cell adhesion molecule is a protein that in humans is encoded by the NRCAM gene. Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. Also, this gene is expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms.
Human Neuronal cell adhesion molecule Protein information
Uniprot ID NRCAM_HUMAN
Uniprot AC Q92823; A4D0S3; E9PDA4; O15051; O15179; Q14BM2; Q9UHI3; Q9UHI4;
UniGene Hs.21422;
GeneID 4897
KEGG hsa:4897;
Human Neuronal cell adhesion molecule Protein SEQUENCE
SEQUENCE 1304 AA; 143890 MW; 1F2698BC7DB5C363 CRC64;
MQLKIMPKKK RLSAGRVPLI LFLCQMISAL EVPLDPKLLE DLVQPPTITQ
QSPKDYIIDP RENIVIQCEA KGKPPPSFSW TRNGTHFDID KDPLVTMKPG
TGTLIINIMS EGKAETYEGV YQCTARNERG AAVSNNIVVR PSRSPLWTKE
KLEPITLQSG QSLVLPCRPP IGLPPPIIFW MDNSFQRLPQ SERVSQGLNG
DLYFSNVLPE DTREDYICYA RFNHTQTIQQ KQPISVKVIS VDELNDTIAA
NLSDTEFYGA KSSRERPPTF LTPEGNASNK EELRGNVLSL ECIAEGLPTP
IIYWAKEDGM LPKNRTVYKN FEKTLQIIHV SEADSGNYQC IAKNALGAIH
HTISVRVKAA PYWITAPQNL VLSPGEDGTL ICRANGNPKP RISWLTNGVP
IEIAPDDPSR KIDGDTIIFS NVQERSSAVY QCNASNEYGY LLANAFVNVL
AEPPRILTPA NTLYQVIANR PALLDCAFFG SPLPTIEWFK GAKGSALHED
IYVLHENGTL EIPVAQKDST GTYTCVARNK LGMAKNEVHL EIKDPTWIVK
QPEYAVVQRG SMVSFECKVK HDHTLSLTVL WLKDNRELPS DERFTVDKDH
LVVADVSDDD SGTYTCVANT TLDSVSASAV LSVVAPTPTP APVYDVPNPP
FDLELTDQLD KSVQLSWTPG DDNNSPITKF IIEYEDAMHK PGLWHHQTEV
SGTQTTAQLK LSPYVNYSFR VMAVNSIGKS LPSEASEQYL TKASEPDKNP
TAVEGLGSEP DNLVITWKPL NGFESNGPGL QYKVSWRQKD GDDEWTSVVV
ANVSKYIVSG TPTFVPYLIK VQALNDMGFA PEPAVVMGHS GEDLPMVAPG
NVRVNVVNST LAEVHWDPVP LKSIRGHLQG YRIYYWKTQS SSKRNRRHIE
KKILTFQGSK THGMLPGLEP FSHYTLNVRV VNGKGEGPAS PDRVFNTPEG
VPSAPSSLKI VNPTLDSLTL EWDPPSHPNG ILTEYTLKYQ PINSTHELGP
LVDLKIPANK TRWTLKNLNF STRYKFYFYA QTSAGSGSQI TEEAVTTVDE
AGILPPDVGA GKVQAVNPRI SNLTAAAAET YANISWEYEG PEHVNFYVEY
GVAGSKEEWR KEIVNGSRSF FGLKGLMPGT AYKVRVGAVG DSGFVSSEDV
FETGPAMASR QVDIATQGWF IGLMCAVALL ILILLIVCFI RRNKGGKYPV
KEKEDAHADP EIQPMKEDDG TFGEYSDAED HKPLKKGSRT PSDRTVKKED
SDDSLVDYGE GVNGQFNEDG SFIGQYSGKK EKEPAEGNES SEAPSPVNAM
NSFV
UCSC uc003vfc.4; human. [Q92823-1];

Category

Human NRCAM ELISA Kit

Human Nr-CAM ELISA Kit;Human neuronal surface protein Bravo ELISA Kit;Human hBravo ELISA Kit;Human NgCAM-related cell adhesion molecule ELISA Kit;Human Ng-CAM-related ELISA Kit;Human KIAA0343 ELISA Kit;Human neuronal cell adhesion molecule ELISA Kit;