Human RNASE2 ELISA Kit

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  • Alternative name

    Human eosinophil-derived neurotoxin ELISA Kit;Human RNase UpI-2 ELISA Kit;Human ribonuclease 2 ELISA Kit;Human RNase 2 ELISA Kit;Human ribonuclease US ELISA Kit;Human EDN ELISA Kit;Human RNS2 ELISA Kit;Human RAF3 ELISA Kit;Human ribonuclease A family member 2 ELISA Kit;Human non-secretory ribonuclease ELISA Kit;Human ribonuclease A F3 ELISA Kit;Human ribonuclease, RNase A family, 2 (liver, eosinophil-derived neurotoxin) ELISA Kit;

  • Catalog
    E026718
  • species
    Human
  • GeneRNASE2
  • Standard CurveHuman RNASE2 ELISA Kit
  • Other Species Mouse Rnase2 ELISA KitMonkey RNASE2 ELISA KitPorcine RNASE2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of RNASE2. No significant cross-reactivity or interference between RNASE2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RNASE2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman RNASE2 ELISA Kit allows for the in vitro quantitative determination of RNASE2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    Intended Uses: This RNASE2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human RNASE2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||RNASE2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-RNASE2 antibody and an RNASE2-HRP conjugate. The assay sample and buffer are incubated together with RNASE2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RNASE2 concentration since RNASE2 from samples and RNASE2-HRP conjugate compete for the anti-RNASE2 antibody binding site. Since the number of sites is limited, as more sites are occupied by RNASE2 from the sample, fewer sites are left to bind RNASE2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RNASE2 concentration in each sample is interpolated from this standard curve.
  • Human Non-secretory ribonuclease Protein information
  • Uniprot ID RNAS2_HUMAN
  • Uniprot AC P10153; Q52M39; Q9H2B7; Q9UCG7;
  • UniGene Hs.728;
  • GeneID 6036
  • KEGG hsa:6036;
  • Human Non-secretory ribonuclease Protein SEQUENCE
  • SEQUENCE 161 AA; 18354 MW; 9406C4596CA69038 CRC64;

    MVPKLFTSQI CLLLLLGLLA VEGSLHVKPP QFTWAQWFET QHINMTSQQC

    TNAMQVINNY QRRCKNQNTF LLTTFANVVN VCGNPNMTCP SNKTRKNCHH

    SGSQVPLIHC NLTTPSPQNI SNCRYAQTPA NMFYIVACDN RDQRRDPPQY

    PVVPVHLDRI I




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