Human NOSIP ELISA Kit

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  • Alternative name

    Human E3 ubiquitin-protein ligase NOSIP ELISA Kit;Human RING-type E3 ubiquitin transferase NOSIP ELISA Kit;Human eNOS-interacting protein ELISA Kit;Human CGI-25 ELISA Kit;Human nitric oxide synthase interacting protein ELISA Kit;Human nitric oxide synthase-interacting protein ELISA Kit;Human RING-type E3 ubiquitin transferase NOSIP ELISA Kit;

  • Catalog
    E026625
  • species
    Human
  • GeneNOSIP
  • Standard CurveHuman NOSIP ELISA Kit
  • Other Species Mouse Nosip ELISA KitBovine NOSIP ELISA KitMonkey NOSIP ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Nitric Oxide Synthase Interacting Protein (NOSIP). No significant cross-reactivity or interference between Nitric Oxide Synthase Interacting Protein (NOSIP) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.053ng/mL.
  • Intended UseHuman NOSIP ELISA Kit allows for the in vitro quantitative determination of NOSIP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nitric Oxide Synthase Interacting Protein (NOSIP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nitric Oxide Synthase Interacting Protein (NOSIP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nitric Oxide Synthase Interacting Protein (NOSIP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nitric Oxide Synthase Interacting Protein (NOSIP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Nitric oxide synthase-interacting protein Protein information
  • Uniprot ID NOSIP_HUMAN
  • Uniprot AC Q9Y314; Q96FD2;
  • UniGene Hs.7236;
  • GeneID 51070
  • KEGG hsa:51070;
  • Human Nitric oxide synthase-interacting protein Protein SEQUENCE
  • SEQUENCE 301 AA; 33172 MW; FB77D6E31511C884 CRC64;

    MTRHGKNCTA GAVYTYHEKK KDTAASGYGT QNIRLSRDAV KDFDCCCLSL

    QPCHDPVVTP DGYLYEREAI LEYILHQKKE IARQMKAYEK QRGTRREEQK

    ELQRAASQDH VRGFLEKESA IVSRPLNPFT AKALSGTSPD DVQPGPSVGP

    PSKDKDKVLP SFWIPSLTPE AKATKLEKPS RTVTCPMSGK PLRMSDLTPV

    HFTPLDSSVD RVGLITRSER YVCAVTRDSL SNATPCAVLR PSGAVVTLEC

    VEKLIRKDMV DPVTGDKLTD RDIIVLQRGG TGFAGSGVKL QAEKSRPVMQ

    A

  • UCSC uc002pol.5; human.;



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