Human NOS3 ELISA Kit

Alternative name
Human constitutive NOS ELISA Kit;Human cNOS ELISA Kit;Human EC-NOS ELISA Kit;Human endothelial NOS ELISA Kit;Human eNOS ELISA Kit;Human NOS type III ELISA Kit;Human NOSIII ELISA Kit;Human ECNOS ELISA Kit;Human nitric oxide synthase 3 ELISA Kit;Human nitric oxide synthase, endothelial ELISA Kit;Human nitric oxide synthase 3 (endothelial cell) ELISA Kit;Human nitric oxide synthase 3 transcript variant eNOS-delta20 ELISA Kit;Human nitric oxide synthase 3 transcript variant eNOS-delta20-21 ELISA Kit;Human nitric oxide synthase 3 transcript variant eNOS-delta21 ELISA Kit;
Catalog
E026622
species
Human
GeneNOS3
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human NOS3. No significant cross-reactivity or interference between Human NOS3 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.27 IU/ml
Intended UseHuman NOS3 ELISA Kit allows for the in vitro quantitative determination of NOS3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for eNOS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any eNOS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for eNOS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of eNOS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Nitric oxide synthase, endothelial Protein information
Uniprot ID NOS3_HUMAN
Uniprot AC Q14434; Q548C1; Q6GSL5; Q9UDC6;
UniGene Hs.647092;
GeneID 4846
KEGG hsa:4846;
Human Nitric oxide synthase, endothelial Protein SEQUENCE
SEQUENCE 1203 AA; 133289 MW; CD9E60FA277B22C6 CRC64;
MGNLKSVAQE PGPPCGLGLG LGLGLCGKQG PATPAPEPSR APASLLPPAP
EHSPPSSPLT QPPEGPKFPR VKNWEVGSIT YDTLSAQAQQ DGPCTPRRCL
GSLVFPRKLQ GRPSPGPPAP EQLLSQARDF INQYYSSIKR SGSQAHEQRL
QEVEAEVAAT GTYQLRESEL VFGAKQAWRN APRCVGRIQW GKLQVFDARD
CRSAQEMFTY ICNHIKYATN RGNLRSAITV FPQRCPGRGD FRIWNSQLVR
YAGYRQQDGS VRGDPANVEI TELCIQHGWT PGNGRFDVLP LLLQAPDEPP
ELFLLPPELV LEVPLEHPTL EWFAALGLRW YALPAVSNML LEIGGLEFPA
APFSGWYMST EIGTRNLCDP HRYNILEDVA VCMDLDTRTT SSLWKDKAAV
EINVAVLHSY QLAKVTIVDH HAATASFMKH LENEQKARGG CPADWAWIVP
PISGSLTPVF HQEMVNYFLS PAFRYQPDPW KGSAAKGTGI TRKKTFKEVA
NAVKISASLM GTVMAKRVKA TILYGSETGR AQSYAQQLGR LFRKAFDPRV
LCMDEYDVVS LEHETLVLVV TSTFGNGDPP ENGESFAAAL MEMSGPYNSS
PRPEQHKSYK IRFNSISCSD PLVSSWRRKR KESSNTDSAG ALGTLRFCVF
GLGSRAYPHF CAFARAVDTR LEELGGERLL QLGQGDELCG QEEAFRGWAQ
AAFQAACETF CVGEDAKAAA RDIFSPKRSW KRQRYRLSAQ AEGLQLLPGL
IHVHRRKMFQ ATIRSVENLQ SSKSTRATIL VRLDTGGQEG LQYQPGDHIG
VCPPNRPGLV EALLSRVEDP PAPTEPVAVE QLEKGSPGGP PPGWVRDPRL
PPCTLRQALT FFLDITSPPS PQLLRLLSTL AEEPREQQEL EALSQDPRRY
EEWKWFRCPT LLEVLEQFPS VALPAPLLLT QLPLLQPRYY SVSSAPSTHP
GEIHLTVAVL AYRTQDGLGP LHYGVCSTWL SQLKPGDPVP CFIRGAPSFR
LPPDPSLPCI LVGPGTGIAP FRGFWQERLH DIESKGLQPT PMTLVFGCRC
SQLDHLYRDE VQNAQQRGVF GRVLTAFSRE PDNPKTYVQD ILRTELAAEV
HRVLCLERGH MFVCGDVTMA TNVLQTVQRI LATEGDMELD EAGDVIGVLR
DQQRYHEDIF GLTLRTQEVT SRIRTQSFSL QERQLRGAVP WAFDPPGSDT
NSP
UCSC uc003wif.4; human. [P29474-1];

Category

Human NOS3 ELISA Kit