Human PLA2 ELISA Kit

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  • Alternative name

    Acidic phospholipase A2 pgPLA 1b/pgPLA 2b ELISA KIT; Phosphatidylcholine 2-acylhydrolasesvPLA2 ELISA KIT; svPLA2 ELISA KIT

  • Catalog
  • species
  • GenePLA2
  • Standard CurveHuman PLA2 ELISA Kit
  • Other Species Human Lp-PLA2 ELISA KitMouse PLA2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PLA2-Ab. No significant cross-reactivity or interference between PLA2-Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PLA2-Ab and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL
  • Intended UseHuman PLA2 ELISA Kit allows for the in vitro quantitative determination of PLA2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology
  • Product Description
    Intended Uses: This PLA2-Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PLA2-Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||PLA2-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing PLA2 antigen and an PLA2-Ab-HRP conjugate. The assay sample and buffer are incubated together with PLA2-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PLA2-Ab concentration since PLA2-Ab from samples and PLA2-Ab-HRP conjugate compete for the PLA2 antigen binding site. Since the number of sites is limited, as more sites are occupied by PLA2-Ab from the sample, fewer sites are left to bind PLA2-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLA2-Ab concentration in each sample is interpolated from this standard curve.

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