Human NOXIN ELISA Kit

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  • Alternative name

    DNA damage-induced apoptosis suppressor protein ELISA KIT; Nitric oxide-inducible gene proteinDDIAS ELISA KIT; C11orf82 ELISA KIT; NOXIN ELISA KIT

  • Catalog
    E026609
  • species
    Human
  • GeneNOXIN
  • Standard CurveHuman NOXIN ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Nitric Oxide Inducible Gene Protein (NOXIN). No significant cross-reactivity or interference between Nitric Oxide Inducible Gene Protein (NOXIN) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.114ng/mL.
  • Intended UseHuman NOXIN ELISA Kit allows for the in vitro quantitative determination of NOXIN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nitric Oxide Inducible Gene Protein (NOXIN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nitric Oxide Inducible Gene Protein (NOXIN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nitric Oxide Inducible Gene Protein (NOXIN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nitric Oxide Inducible Gene Protein (NOXIN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.




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