Human NISCH ELISA Kit

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  • Alternative name

    Human imidazoline receptor 1 ELISA Kit;Human I-1 ELISA Kit;Human IR1 ELISA Kit;Human Imidazoline receptor antisera-selected protein ELISA Kit;Human hIRAS ELISA Kit;Human Imidazoline-1 receptor ELISA Kit;Human I1R ELISA Kit;Human Imidazoline-1 receptor candidate protein ELISA Kit;Human I-1 receptor candidate protein ELISA Kit;Human I1R candidate protein ELISA Kit;Human IRAS ELISA Kit;Human KIAA0975 ELISA Kit;Human nischarin ELISA Kit;Human imidazoline receptor antisera selected ELISA Kit;

  • Catalog
    E026605
  • species
    Human
  • GeneNISCH
  • Standard CurveHuman NISCH ELISA Kit
  • Other Species Mouse Nisch ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Nischarin (NISCH). No significant cross-reactivity or interference between Nischarin (NISCH) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.054ng/mL.
  • Intended UseHuman NISCH ELISA Kit allows for the in vitro quantitative determination of NISCH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nischarin (NISCH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nischarin (NISCH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nischarin (NISCH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nischarin (NISCH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Nischarin Protein information
  • Uniprot ID NISCH_HUMAN
  • Uniprot AC Q9UFW3;
  • UniGene Hs.435290;
  • GeneID 11188
  • KEGG hsa:11188;
  • Human Nischarin Protein SEQUENCE
  • SEQUENCE 1504 AA; 166629 MW; 7761C7DA1FDC4E53 CRC64;

    MATARTFGPE REAEPAKEAR VVGSELVDTY TVYIIQVTDG SHEWTVKHRY

    SDFHDLHEKL VAERKIDKNL LPPKKIIGKN SRSLVEKREK DLEVYLQKLL

    AAFPGVTPRV LAHFLHFHFY EINGITAALA EELFEKGEQL LGAGEVFAIG

    PLQLYAVTEQ LQQGKPTCAS GDAKTDLGHI LDFTCRLKYL KVSGTEGPFG

    TSNIQEQLLP FDLSIFKSLH QVEISHCDAK HIRGLVASKP TLATLSVRFS

    ATSMKEVLVP EASEFDEWEP EGTTLEGPVT AVIPTWQALT TLDLSHNSVS

    EIDESVKLIP KIEFLDLSHN GLLVVDNLQH LYNLVHLDLS YNKLSSLEGL

    HTKLGNIKTL NLAGNLLESL SGLHKLYSLV NLDLRDNRIE QMEEVRSIGS

    LPCLEHVSLL NNPLSIIPDY RTKVLAQFGE RASEVCLDDT VTTEKELDTV

    EVLKAIQKAK EVKSKLSNPE KKGGEDSRLS AAPCIRPSSS PPTVAPASAS

    LPQPILSNQG IMFVQEEALA SSLSSTDSLT PEHQPIAQGC SDSLESIPAG

    QAASDDLRDV PGAVGGASPE HAEPEVQVVP GSGQIIFLPF TCIGYTATNQ

    DFIQRLSTLI RQAIERQLPA WIEAANQREE GQGEQGEEED EEEEEEEDVA

    ENRYFEMGPP DVEEEEGGGQ GEEEEEEEED EEAEEERLAL EWALGADEDF

    LLEHIRILKV LWCFLIHVQG SIRQFAACLV LTDFGIAVFE IPHQESRGSS

    QHILSSLRFV FCFPHGDLTE FGFLMPELCL VLKVRHSENT LFIISDAANL

    HEFHADLRSC FAPQHMAMLC SPILYGSHTS LQEFLRQLLT FYKVAGGCQE

    RSQGCFPVYL VYSDKRMVQT AAGDYSGNIE WASCTLCSAV RRSCCAPSEA

    VKSAAIPYWL LLTPQHLNVI KADFNPMPNR GTHNCRNRNS FKLSRVPLST

    VLLDPTRSCT QPRGAFADGH VLELLVGYRF VTAIFVLPHE KFHFLRVYNQ

    LRASLQDLKT VVIAKTPGTG GSPQGSFADG QPAERRASND QRPQEVPAEA

    LAPAPAEVPA PAPAAASASG PAKTPAPAEA STSALVPEET PVEAPAPPPA

    EAPAQYPSEH LIQATSEENQ IPSHLPACPS LRHVASLRGS AIIELFHSSI

    AEVENEELRH LMWSSVVFYQ TPGLEVTACV LLSTKAVYFV LHDGLRRYFS

    EPLQDFWHQK NTDYNNSPFH ISQCFVLKLS DLQSVNVGLF DQHFRLTGST

    PMQVVTCLTR DSYLTHCFLQ HLMVVLSSLE RTPSPEPVDK DFYSEFGNKT

    TGKMENYELI HSSRVKFTYP SEEEIGDLTF TVAQKMAEPE KAPALSILLY

    VQAFQVGMPP PGCCRGPLRP KTLLLTSSEI FLLDEDCVHY PLPEFAKEPP

    QRDRYRLDDG RRVRDLDRVL MGYQTYPQAL TLVFDDVQGH DLMGSVTLDH

    FGEVPGGPAR ASQGREVQWQ VFVPSAESRE KLISLLARQW EALCGRELPV

    ELTG

  • UCSC uc003ded.5; human. [Q9Y2I1-1];


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