Human NID1 ELISA Kit

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  • Alternative name

    Human NID-1 ELISA Kit;Human entactin ELISA Kit;Human NID ELISA Kit;Human nidogen 1 ELISA Kit;Human nidogen-1 ELISA Kit;Human enactin ELISA Kit;

  • Catalog
    E026554
  • species
    Human
  • GeneNID1
  • Other Species Mouse Nid1 ELISA KitRat Nid1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human NID 1. No significant cross-reactivity or interference between human NID 1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human NID 1 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m
  • Intended UseHuman NID1 ELISA Kit allows for the in vitro quantitative determination of NID1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NID 1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NID 1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NID 1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NID 1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Nidogen-1 Protein information
  • Uniprot ID NID1_HUMAN
  • Uniprot AC P14543; Q14942; Q59FL2; Q5TAF2; Q5TAF3; Q86XD7;
  • UniGene Hs.356624;
  • GeneID 4811
  • KEGG hsa:4811;
  • Human Nidogen-1 Protein SEQUENCE
  • SEQUENCE 1247 AA; 136377 MW; FCC839416525CD48 CRC64;

    MLASSSRIRA AWTRALLLPL LLAGPVGCLS RQELFPFGPG QGDLELEDGD

    DFVSPALELS GALRFYDRSD IDAVYVTTNG IIATSEPPAK ESHPGLFPPT

    FGAVAPFLAD LDTTDGLGKV YYREDLSPSI TQRAAECVHR GFPEISFQPS

    SAVVVTWESV APYQGPSRDP DQKGKRNTFQ AVLASSDSSS YAIFLYPEDG

    LQFHTTFSKK ENNQVPAVVA FSQGSVGFLW KSNGAYNIFA NDRESVENLA

    KSSNSGQQGV WVFEIGSPAT TNGVVPADVI LGTEDGAEYD DEDEDYDLAT

    TRLGLEDVGT TPFSYKALRR GGADTYSVPS VLSPRRAATE RPLGPPTERT

    RSFQLAVETF HQQHPQVIDV DEVEETGVVF SYNTDSRQTC ANNRHQCSVH

    AECRDYATGF CCSCVAGYTG NGRQCVAEGS PQRVNGKVKG RIFVGSSQVP

    IVFENTDLHS YVVMNHGRSY TAISTIPETV GYSLLPLAPV GGIIGWMFAV

    EQDGFKNGFS ITGGEFTRQA EVTFVGHPGN LVIKQRFSGI DEHGHLTIDT

    ELEGRVPQIP FGSSVHIEPY TELYHYSTSV ITSSSTREYT VTEPERDGAS

    PSRIYTYQWR QTITFQECVH DDSRPALPST QQLSVDSVFV LYNQEEKILR

    YALSNSIGPV REGSPDALQN PCYIGTHGCD TNAACRPGPR TQFTCECSIG

    FRGDGRTCYD IDECSEQPSV CGSHTICNNH PGTFRCECVE GYQFSDEGTC

    VAVVDQRPIN YCETGLHNCD IPQRAQCIYT GGSSYTCSCL PGFSGDGQAC

    QDVDECQPSR CHPDAFCYNT PGSFTCQCKP GYQGDGFRCV PGEVEKTRCQ

    HEREHILGAA GATDPQRPIP PGLFVPECDA HGHYAPTQCH GSTGYCWCVD

    RDGREVEGTR TRPGMTPPCL STVAPPIHQG PAVPTAVIPL PPGTHLLFAQ

    TGKIERLPLE GNTMRKTEAK AFLHVPAKVI IGLAFDCVDK MVYWTDITEP

    SIGRASLHGG EPTTIIRQDL GSPEGIAVDH LGRNIFWTDS NLDRIEVAKL

    DGTQRRVLFE TDLVNPRGIV TDSVRGNLYW TDWNRDNPKI ETSYMDGTNR

    RILVQDDLGL PNGLTFDAFS SQLCWVDAGT NRAECLNPSQ PSRRKALEGL

    QYPFAVTSYG KNLYFTDWKM NSVVALDLAI SKETDAFQPH KQTRLYGITT

    ALSQCPQGHN YCSVNNGGCT HLCLATPGSR TCRCPDNTLG VDCIEQK

  • UCSC uc001hxo.4; human. [P14543-1];


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