Human NNMT ELISA Kit

Alternative name
Human nicotinamide N-methyltransferase ELISA Kit;
Catalog
E026534
species
Human
GeneNNMT
SpecificityThis assay has high sensitivity and excellent specificity for detection of human NNMT. No significant cross-reactivity or interference between human NNMT and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human NNMT is typically less than 7 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O
Intended UseHuman NNMT ELISA Kit allows for the in vitro quantitative determination of NNMT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NNMT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NNMT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NNMT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NNMT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Nicotinamide N-methyltransferase Protein information
Uniprot ID NNMT_HUMAN
Uniprot AC P40261;
UniGene Hs.503911;
GeneID 4837
KEGG hsa:4837;
Human Nicotinamide N-methyltransferase Protein SEQUENCE
SEQUENCE 264 AA; 29574 MW; 280B12748F4488AC CRC64;
MESGFTSKDT YLSHFNPRDY LEKYYKFGSR HSAESQILKH LLKNLFKIFC
LDGVKGDLLI DIGSGPTIYQ LLSACESFKE IVVTDYSDQN LQELEKWLKK
EPEAFDWSPV VTYVCDLEGN RVKGPEKEEK LRQAVKQVLK CDVTQSQPLG
AVPLPPADCV LSTLCLDAAC PDLPTYCRAL RNLGSLLKPG GFLVIMDALK
SSYYMIGEQK FSSLPLGREA VEAAVKEAGY TIEWFEVISQ SYSSTMANNE
GLFSLVARKL SRPL

Category

Human NNMT ELISA Kit

Human nicotinamide N-methyltransferase ELISA Kit;