Human NNMT ELISA Kit

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  • Alternative name

    Human nicotinamide N-methyltransferase ELISA Kit;

  • Catalog
    E026534
  • species
    Human
  • GeneNNMT
  • Other Species Mouse Nnmt ELISA KitPorcine NNMT ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human NNMT. No significant cross-reactivity or interference between human NNMT and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human NNMT is typically less than 7 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O
  • Intended UseHuman NNMT ELISA Kit allows for the in vitro quantitative determination of NNMT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NNMT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NNMT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NNMT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NNMT bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Nicotinamide N-methyltransferase Protein information
  • Uniprot ID NNMT_HUMAN
  • Uniprot AC P40261;
  • UniGene Hs.503911;
  • GeneID 4837
  • KEGG hsa:4837;
  • Human Nicotinamide N-methyltransferase Protein SEQUENCE
  • SEQUENCE 264 AA; 29574 MW; 280B12748F4488AC CRC64;

    MESGFTSKDT YLSHFNPRDY LEKYYKFGSR HSAESQILKH LLKNLFKIFC

    LDGVKGDLLI DIGSGPTIYQ LLSACESFKE IVVTDYSDQN LQELEKWLKK

    EPEAFDWSPV VTYVCDLEGN RVKGPEKEEK LRQAVKQVLK CDVTQSQPLG

    AVPLPPADCV LSTLCLDAAC PDLPTYCRAL RNLGSLLKPG GFLVIMDALK

    SSYYMIGEQK FSSLPLGREA VEAAVKEAGY TIEWFEVISQ SYSSTMANNE

    GLFSLVARKL SRPL



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