Human NOX4 ELISA Kit

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  • Alternative name

    Human kidney oxidase-1 ELISA Kit;Human KOX-1 ELISA Kit;Human kidney superoxide-producing NADPH oxidase ELISA Kit;Human renal NAD(P)H-oxidase ELISA Kit;Human RENOX ELISA Kit;Human KOX ELISA Kit;Human NADPH oxidase 4 ELISA Kit;

  • Catalog
    E026523
  • species
    Human
  • GeneNOX4
  • Standard CurveHuman NOX4 ELISA Kit
  • Other Species Mouse Nox4 ELISA KitRat Nox4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4). No significant cross-reactivity or interference between Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.057ng/mL.
  • Intended UseHuman NOX4 ELISA Kit allows for the in vitro quantitative determination of NOX4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human NADPH oxidase 4 Protein information
  • Uniprot ID NOX4_HUMAN
  • Uniprot AC Q7Z7G3; Q86V92;
  • UniGene Hs.371036;
  • GeneID 50507
  • KEGG hsa:50507;
  • Human NADPH oxidase 4 Protein SEQUENCE
  • SEQUENCE 578 AA; 66932 MW; D150A92CC71DD40D CRC64;

    MAVSWRSWLA NEGVKHLCLF IWLSMNVLLF WKTFLLYNQG PEYHYLHQML

    GLGLCLSRAS ASVLNLNCSL ILLPMCRTLL AYLRGSQKVP SRRTRRLLDK

    SRTFHITCGV TICIFSGVHV AAHLVNALNF SVNYSEDFVE LNAARYRDED

    PRKLLFTTVP GLTGVCMVVV LFLMITASTY AIRVSNYDIF WYTHNLFFVF

    YMLLTLHVSG GLLKYQTNLD THPPGCISLN RTSSQNISLP EYFSEHFHEP

    FPEGFSKPAE FTQHKFVKIC MEEPRFQANF PQTWLWISGP LCLYCAERLY

    RYIRSNKPVT IISVMSHPSD VMEIRMVKEN FKARPGQYIT LHCPSVSALE

    NHPFTLTMCP TETKATFGVH LKIVGDWTER FRDLLLPPSS QDSEILPFIQ

    SRNYPKLYID GPFGSPFEES LNYEVSLCVA GGIGVTPFAS ILNTLLDDWK

    PYKLRRLYFI WVCRDIQSFR WFADLLCMLH NKFWQENRPD YVNIQLYLSQ

    TDGIQKIIGE KYHALNSRLF IGRPRWKLLF DEIAKYNRGK TVGVFCCGPN

    SLSKTLHKLS NQNNSYGTRF EYNKESFS

  • UCSC uc001pct.4; human. [Q9NPH5-1];



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