Human NOX1 ELISA Kit

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  • Alternative name

    Human NOX-1 ELISA Kit;Human mitogenic oxidase 1 ELISA Kit;Human MOX-1 ELISA Kit;Human NADH/NADPH mitogenic oxidase subunit P65-MOX ELISA Kit;Human NOH-1 ELISA Kit;Human MOX1 ELISA Kit;Human NOH1 ELISA Kit;Human GP91-2 ELISA Kit;Human NADPH oxidase 1 ELISA Kit;Human NADPH oxidase homolog-1 ELISA Kit;Human mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating) ELISA Kit;

  • Catalog
    E026517
  • species
    Human
  • GeneNOX1
  • Standard CurveHuman NOX1 ELISA Kit
  • Other Species Mouse Nox1 ELISA KitRat Nox1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1). No significant cross-reactivity or interference between Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.061ng/mL.
  • Intended UseHuman NOX1 ELISA Kit allows for the in vitro quantitative determination of NOX1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human NADPH oxidase 1 Protein information
  • Uniprot ID NOX1_HUMAN
  • Uniprot AC Q9Y5S8; A8K836; O95691; Q2PP02;
  • UniGene Hs.592227;
  • GeneID 27035
  • KEGG hsa:27035;
  • Human NADPH oxidase 1 Protein SEQUENCE
  • SEQUENCE 564 AA; 64871 MW; C3BE290F4E6DBC9A CRC64;

    MGNWVVNHWF SVLFLVVWLG LNVFLFVDAF LKYEKADKYY YTRKILGSTL

    ACARASALCL NFNSTLILLP VCRNLLSFLR GTCSFCSRTL RKQLDHNLTF

    HKLVAYMICL HTAIHIIAHL FNFDCYSRSR QATDGSLASI LSSLSHDEKK

    GGSWLNPIQS RNTTVEYVTF TSIAGLTGVI MTIALILMVT SATEFIRRSY

    FEVFWYTHHL FIFYILGLGI HGIGGIVRGQ TEESMNESHP RKCAESFEMW

    DDRDSHCRRP KFEGHPPESW KWILAPVILY ICERILRFYR SQQKVVITKV

    VMHPSKVLEL QMNKRGFSME VGQYIFVNCP SISLLEWHPF TLTSAPEEDF

    FSIHIRAAGD WTENLIRAFE QQYSPIPRIE VDGPFGTASE DVFQYEVAVL

    VGAGIGVTPF ASILKSIWYK FQCADHNLKT KKIYFYWICR ETGAFSWFNN

    LLTSLEQEME ELGKVGFLNY RLFLTGWDSN IVGHAALNFD KATDIVTGLK

    QKTSFGRPMW DNEFSTIATS HPKSVVGVFL CGPRTLAKSL RKCCHRYSSL

    DPRKVQFYFN KENF

  • UCSC uc004egj.3; human. [Q9Y5S8-1];


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