Human NKAP ELISA Kit

Alternative name
Human NFKB activating protein ELISA Kit;Human NF-kappa-B-activating protein ELISA Kit;Human NF-kappaB activating protein ELISA Kit;
Catalog
E026475
species
Human
GeneNKAP
SpecificityThis assay has high sensitivity and excellent specificity for detection of NKAP. No significant cross-reactivity or interference between NKAP and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of NKAP is typically less than 0.065ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two
Intended UseHuman NKAP ELISA Kit allows for the in vitro quantitative determination of NKAP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NKAP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NKAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NKAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NKAP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human NF-kappa-B-activating protein Protein information
Uniprot ID NKAP_HUMAN
Uniprot AC Q8N5F7; Q6IPW6; Q96BQ2; Q9H638;
UniGene Hs.522771;
GeneID 79576
KEGG hsa:79576;
Human NF-kappa-B-activating protein Protein SEQUENCE
SEQUENCE 415 AA; 47138 MW; 44BD1DE20F7ED35A CRC64;
MAPVSGSRSP DREASGSGGR RRSSSKSPKP SKSARSPRGR RSRSHSCSRS
GDRNGLTHQL GGLSQGSRNQ SYRSRSRSRS RERPSAPRGI PFASASSSVY
YGSYSRPYGS DKPWPSLLDK EREESLRQKR LSERERIGEL GAPEVWGLSP
KNPEPDSDEH TPVEDEEPKK STTSASTSEE EKKKKSSRSK ERSKKRRKKK
SSKRKHKKYS EDSDSDSDSE TDSSDEDNKR RAKKAKKKEK KKKHRSKKYK
KKRSKKSRKE SSDSSSKESQ EEFLENPWKD RTKAEEPSDL IGPEAPKTLT
SQDDKPLNYG HALLPGEGAA MAEYVKAGKR IPRRGEIGLT SEEIASFECS
GYVMSGSRHR RMEAVRLRKE NQIYSADEKR ALASFNQEER RKRENKILAS
FREMVYRKTK GKDDK
UCSC uc004esh.4; human.;

Category

Human NKAP ELISA Kit

Human NFKB activating protein ELISA Kit;Human NF-kappa-B-activating protein ELISA Kit;Human NF-kappaB activating protein ELISA Kit;