Human NCF1 ELISA Kit

Alternative name
Human NCF-1 ELISA Kit;Human 47 kDa autosomal chronic granulomatous disease protein ELISA Kit;Human 47 kDa neutrophil oxidase factor ELISA Kit;Human NCF-47K ELISA Kit;Human neutrophil NADPH oxidase factor 1 ELISA Kit;Human nox organizer 2 ELISA Kit;Human nox-organizing protein 2 ELISA Kit;Human SH3 and PX domain-containing protein 1A ELISA Kit;Human p47-phox ELISA Kit;Human NOXO2 ELISA Kit;Human SH3PXD1A ELISA Kit;Human NCF1A ELISA Kit;Human p47phox ELISA Kit;Human neutrophil cytosolic factor 1 ELISA Kit;Human neutrophil cytosol factor 1 ELISA Kit;Human NADPH oxidase organizer 2 ELISA Kit;Human neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) ELISA Kit;
Catalog
E026421
species
Human
GeneNCF1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Neutrophil Cytosolic Factor 1 (NCF1). No significant cross-reactivity or interference between Neutrophil Cytosolic Factor 1 (NCF1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.113ng/mL.
Intended UseHuman NCF1 ELISA Kit allows for the in vitro quantitative determination of NCF1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutrophil Cytosolic Factor 1 (NCF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neutrophil Cytosolic Factor 1 (NCF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neutrophil Cytosolic Factor 1 (NCF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neutrophil Cytosolic Factor 1 (NCF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Neutrophil cytosol factor 1 Protein information
Uniprot ID NCF1_HUMAN
Uniprot AC Q9BXI8; Q9UDV9; Q9UMU2;
UniGene Hs.647047; Hs.648940; Hs.655201;
GeneID 653361
KEGG hsa:653361;
Human Neutrophil cytosol factor 1 Protein SEQUENCE
SEQUENCE 390 AA; 44652 MW; 5A0F9F0EF101D2DB CRC64;
MGDTFIRHIA LLGFEKRFVP SQHYVYMFLV KWQDLSEKVV YRRFTEIYEF
HKTLKEMFPI EAGAINPENR IIPHLPAPKW FDGQRAAENR QGTLTEYCGT
LMSLPTKISR CPHLLDFFKV RPDDLKLPTD NQTKKPETYL MPKDGKSTAT
DITGPIILQT YRAIANYEKT SGSEMALSTG DVVEVVEKSE SGWWFCQMKA
KRGWIPASFL EPLDSPDETE DPEPNYAGEP YVAIKAYTAV EGDEVSLLEG
EAVEVIHKLL DGWWVIRKDD VTGYFPSMYL QKSGQDVSQA QRQIKRGAPP
RRSSIRNAHS IHQRSRKRLS QDAYRRNSVR FLQQRRRQAR PGPQSPGSPL
EEERQTQRSK PQPAVPPRPS ADLILNRCSE STKRKLASAV
UCSC uc003ubb.5; human. [P14598-1];

Category

Human NCF1 ELISA Kit