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Human NSMAF ELISA Kit

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  • Alternative name

    Human factor associated with neutral sphingomyelinase activation ELISA Kit;Human factor associated with N-SMase activation ELISA Kit;Human FAN ELISA Kit;Human GRAMD5 ELISA Kit;Human neutral sphingomyelinase activation associated factor ELISA Kit;Human protein FAN ELISA Kit;Human neutral sphingomyelinase (N-SMase) activation associated factor ELISA Kit;
  • Catalog
    E026405
  • species
    Human
  • GeneNSMAF
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Neutral Sphingomyelinase Activation Associated Factor (NSMAF). No significant cross-reactivity or interference between Neutral Sphingomyelinase Activation Associated Factor (NSMAF) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.054ng/mL.
  • Intended UseHuman NSMAF ELISA Kit allows for the in vitro quantitative determination of NSMAF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutral Sphingomyelinase Activation Associated Factor (NSMAF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neutral Sphingomyelinase Activation Associated Factor (NSMAF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neutral Sphingomyelinase Activation Associated Factor (NSMAF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neutral Sphingomyelinase Activation Associated Factor (NSMAF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Protein FAN Protein information
  • Uniprot ID FAN_HUMAN
  • Uniprot AC Q92636; B4DFB0; E9PCH0; Q8IW26;
  • UniGene Hs.372000;
  • GeneID 8439
  • KEGG hsa:8439;
  • Human Protein FAN Protein SEQUENCE

  • SEQUENCE 917 AA; 104372 MW; 36498B5A1378E49C CRC64;

    MAFIRKKQQE QQLQLYSKER FSLLLLNLEE YYFEQHRANH ILHKGSHHER

    KIRGSLKICS KSVIFEPDSI SQPIIKIPLR DCIKIGKHGE NGANRHFTKA

    KSGGISLIFS QVYFIKEHNV VAPYKIERGK MEYVFELDVP GKVEDVVETL

    LQLHRASCLD KLGDQTAMIT AILQSRLART SFDKNRFQNI SEKLHMECKA

    EMVTPLVTNP GHVCITDTNL YFQPLNGYPK PVVQITLQDV RRIYKRRHGL

    MPLGLEVFCT EDDLCSDIYL KFYEPQDRDD LYFYIATYLE HHVAEHTAES

    YMLQWQRGHL SNYQYLLHLN NLADRSCNDL SQYPVFPWII HDYSSSELDL

    SNPGTFRDLS KPVGALNKER LERLLTRYQE MPEPKFMYGS HYSSPGYVLF

    YLVRIAPEYM LCLQNGRFDN ADRMFNSIAE TWKNCLDGAT DFKELIPEFY

    GDDVSFLVNS LKLDLGKRQG GQMVDDVELP PWASSPEDFL QKSKDALESN

    YVSEHLHEWI DLIFGYKQKG SDAVGAHNVF HPLTYEGGVD LNSIQDPDEK

    VAMLTQILEF GQTPKQLFVT PHPRRITPKF KSLSQTSSYN ASMADSPGEE

    SFEDLTEESK TLAWNNITKL QLHEHYKIHK EAVTGITVSR NGSSVFTTSQ

    DSTLKMFSKE SKMLQRSISF SNMALSSCLL LPGDATVITS SWDNNVYFYS

    IAFGRRQDTL MGHDDAVSKI CWHDNRLYSA SWDSTVKVWS GVPAEMPGTK

    RHHFDLLAEL EHDVSVDTIS LNAASTLLVS GTKEGTVNIW DLTTATLMHQ

    IPCHSGIVCD TAFSPDSRHV LSTGTDGCLN VIDVQTGMLI SSMTSDEPQR

    CFVWDGNSVL SGSQSGELLV WDLLGAKISE RIQGHTGAVT CIWMNEQCSS

    IITGGEDRQI IFWKLQY

  • UCSC uc003xtt.4; human. [Q92636-1];



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