Human NTF3 ELISA Kit

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  • Alternative name

    Human NT-3 ELISA Kit;Human HDNF ELISA Kit;Human nerve growth factor 2 ELISA Kit;Human NGF-2 ELISA Kit;Human neurotrophic factor ELISA Kit;Human NGF2 ELISA Kit;Human NT3 ELISA Kit;Human neurotrophin 3 ELISA Kit;Human neurotrophin-3 ELISA Kit;

  • Catalog
    E026390
  • species
    Human
  • GeneNTF3
  • Standard CurveHuman NTF3 ELISA Kit
  • Other Species Mouse Ntf3 ELISA KitBovine NTF3 ELISA KitChicken NTF3 ELISA KitPorcine NTF3 ELISA KitRat Ntf3 ELISA Kit
  • SpecificityNatural and recombinant human Neurotrophin-3
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 2pg/ml
  • Intended UseHuman NTF3 ELISA Kit allows for the in vitro quantitative determination of NTF3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human Neurotrophin-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Neurotrophin-3 has been precoated onto 96-well plates. Standards(sf21,Y139-T257) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neurotrophin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Neurotrophin-3 amount of sample captured in plate. Background: Neurotrophin-3 (NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3 (Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo.4 NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.
  • Human Neurotrophin-3 Protein information
  • Uniprot ID NTF3_HUMAN
  • Uniprot AC P20783; B7Z1T5; Q6FH50;
  • UniGene Hs.99171;
  • GeneID 4908
  • KEGG hsa:4908;
  • Human Neurotrophin-3 Protein SEQUENCE
  • SEQUENCE 257 AA; 29355 MW; 39A5BB3B28E25E03 CRC64;

    MSILFYVIFL AYLRGIQGNN MDQRSLPEDS LNSLIIKLIQ ADILKNKLSK

    QMVDVKENYQ STLPKAEAPR EPERGGPAKS AFQPVIAMDT ELLRQQRRYN

    SPRVLLSDST PLEPPPLYLM EDYVGSPVVA NRTSRRKRYA EHKSHRGEYS

    VCDSESLWVT DKSSAIDIRG HQVTVLGEIK TGNSPVKQYF YETRCKEARP

    VKNGCRGIDD KHWNSQCKTS QTYVRALTSE NNKLVGWRWI RIDTSCVCAL

    SRKIGRT

  • UCSC uc001qnk.5; human. [P20783-1];



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