Human MAG ELISA Kit

Alternative name
Human SIGLEC-4A ELISA Kit;Human GMA ELISA Kit;Human S-MAG ELISA Kit;Human SIGLEC4A ELISA Kit;Human SPG75 ELISA Kit;Human myelin associated glycoprotein ELISA Kit;Human myelin-associated glycoprotein ELISA Kit;Human sialic acid binding Ig-like lectin 4A ELISA Kit;Human sialic acid-binding immunoglobulin-like lectin 4A ELISA Kit;
Catalog
E002638
species
Human
GeneMAG
Standard Curve
Other Species Human MAG Ab ELISA Kit Mouse Mag ELISA Kit Rat Mag ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Anti-MAG. No significant cross-reactivity or interference between Anti-MAG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Anti-MAG and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman MAG ELISA Kit allows for the in vitro quantitative determination of MAG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalIntended Uses||This Anti-MAG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat Anti-MAG. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: Anti-MAG ELISA kit applies the competitive enzyme immunoassay technique utilizing Myelin Associated Glycoprotein antigen and an Anti-MAG-HRP conjugate. The assay sample and buffer are incubated together with Anti-MAG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-MAG concentration since Anti-MAG from samples and Anti-MAG-HRP conjugate compete for the Myelin Associated Glycoprotein antigen binding site. Since the number of sites is limited, as more sites are occupied by Anti-MAG from the sample, fewer sites are left to bind Anti-MAG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-MAG concentration in each sample is interpolated from this standard curve.
Human Myelin-associated glycoprotein Protein information
Uniprot ID MAG_HUMAN
Uniprot AC P20916; B7Z2E5; F5GYC0; Q567S4;
UniGene Hs.643440;
GeneID 4099
KEGG hsa:4099;
Human Myelin-associated glycoprotein Protein SEQUENCE
SEQUENCE 626 AA; 69069 MW; ED2D36B24F21CAAA CRC64;
MIFLTALPLF WIMISASRGG HWGAWMPSSI SAFEGTCVSI PCRFDFPDEL
RPAVVHGVWY FNSPYPKNYP PVVFKSRTQV VHESFQGRSR LLGDLGLRNC
TLLLSNVSPE LGGKYYFRGD LGGYNQYTFS EHSVLDIVNT PNIVVPPEVV
AGTEVEVSCM VPDNCPELRP ELSWLGHEGL GEPAVLGRLR EDEGTWVQVS
LLHFVPTREA NGHRLGCQAS FPNTTLQFEG YASMDVKYPP VIVEMNSSVE
AIEGSHVSLL CGADSNPPPL LTWMRDGTVL REAVAESLLL ELEEVTPAED
GVYACLAENA YGQDNRTVGL SVMYAPWKPT VNGTMVAVEG ETVSILCSTQ
SNPDPILTIF KEKQILSTVI YESELQLELP AVSPEDDGEY WCVAENQYGQ
RATAFNLSVE FAPVLLLESH CAAARDTVQC LCVVKSNPEP SVAFELPSRN
VTVNESEREF VYSERSGLVL TSILTLRGQA QAPPRVICTA RNLYGAKSLE
LPFQGAHRLM WAKIGPVGAV VAFAILIAIV CYITQTRRKK NVTESPSFSA
GDNPPVLFSS DFRISGAPEK YESERRLGSE RRLLGLRGEP PELDLSYSHS
DLGKRPTKDS YTLTEELAEY AEIRVK
UCSC uc002nyx.3; human. [P20916-1];

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Human MAG ELISA Kit