Human NT 3 ELISA Kit

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  • Alternative name

    Neurotrophin-3 ELISA KIT; HDNF ELISA KIT; Nerve growth factor 2 ELISA KIT; NGF-2 ELISA KIT; Neurotrophic factorNTF3 ELISA KIT; NT-3 ELISA KIT; NGF-2 ELISA KIT

  • Catalog
  • species
  • GeneNT 3
  • Other Species Human NT-3 ELISA KitMouse NT-3 ELISA KitHuman 3 NT ELISA KitHuman 3-NT ELISA KitMouse 3-NT ELISA KitBovine 3-NT ELISA KitCanine 3-NT ELISA KitChicken 3-NT ELISA KitGeneral 3-NT ELISA KitPorcine 3-NT ELISA KitRabbit 3-NT ELISA KitRat 3-NT ELISA KitCamel 3-NT ELISA KitGoat 3-NT ELISA KitGuinea Pig 3-NT ELISA KitHamster 3-NT ELISA KitHorse 3-NT ELISA KitMonkey 3-NT ELISA KitSheep 3-NT ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman NT 3 ELISA Kit allows for the in vitro quantitative determination of NT 3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyNeurobiology
  • Product Description
    Principle of the Assay: NT-3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NT-3 antibody and an NT-3-HRP conjugate. The assay sample and buffer are incubated together with NT-3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NT-3 concentration since NT-3 from samples and NT-3-HRP conjugate compete for the anti-NT-3 antibody binding site. Since the number of sites is limited, as more sites are occupied by NT-3 from the sample, fewer sites are left to bind NT-3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NT-3 concentration in each sample is interpolated from this standard curve.

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