Human NTRK2 ELISA Kit

Alternative name
Human GP145-TrkB ELISA Kit;Human trk-B ELISA Kit;Human neurotrophic tyrosine kinase receptor type 2 ELISA Kit;Human TrkB tyrosine kinase ELISA Kit;Human tropomyosin-related kinase B ELISA Kit;Human TRKB ELISA Kit;Human neurotrophic receptor tyrosine kinase 2 ELISA Kit;Human BDNF/NT-3 growth factors receptor ELISA Kit;Human BDNF-tropomyosine receptor kinase B ELISA Kit;Human tyrosine kinase receptor B ELISA Kit;
Catalog
E026365
species
Human
GeneNTRK2
SpecificityThis assay has high sensitivity and excellent specificity for detection of NTRK2. No significant cross-reactivity or interference between NTRK2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NTRK2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman NTRK2 ELISA Kit allows for the in vitro quantitative determination of NTRK2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalIntended Uses: This NTRK2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NTRK2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||NTRK2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NTRK2 antibody and an NTRK2-HRP conjugate. The assay sample and buffer are incubated together with NTRK2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NTRK2 concentration since NTRK2 from samples and NTRK2-HRP conjugate compete for the anti-NTRK2 antibody binding site. Since the number of sites is limited, as more sites are occupied by NTRK2 from the sample, fewer sites are left to bind NTRK2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NTRK2 concentration in each sample is interpolated from this standard curve.
Human BDNF/NT-3 growth factors receptor Protein information
Uniprot ID NTRK2_HUMAN
Uniprot AC Q16620; B1ANZ4; B4DFV9; Q16675; Q59GJ1; Q8WXJ5; Q8WXJ6; Q8WXJ7;
UniGene Hs.494312; Hs.712776;
GeneID 4915
KEGG hsa:4915;
Human BDNF/NT-3 growth factors receptor Protein SEQUENCE
SEQUENCE 822 AA; 91999 MW; 2FEB9159948F0D13 CRC64;
MSSWIRWHGP AMARLWGFCW LVVGFWRAAF ACPTSCKCSA SRIWCSDPSP
GIVAFPRLEP NSVDPENITE IFIANQKRLE IINEDDVEAY VGLRNLTIVD
SGLKFVAHKA FLKNSNLQHI NFTRNKLTSL SRKHFRHLDL SELILVGNPF
TCSCDIMWIK TLQEAKSSPD TQDLYCLNES SKNIPLANLQ IPNCGLPSAN
LAAPNLTVEE GKSITLSCSV AGDPVPNMYW DVGNLVSKHM NETSHTQGSL
RITNISSDDS GKQISCVAEN LVGEDQDSVN LTVHFAPTIT FLESPTSDHH
WCIPFTVKGN PKPALQWFYN GAILNESKYI CTKIHVTNHT EYHGCLQLDN
PTHMNNGDYT LIAKNEYGKD EKQISAHFMG WPGIDDGANP NYPDVIYEDY
GTAANDIGDT TNRSNEIPST DVTDKTGREH LSVYAVVVIA SVVGFCLLVM
LFLLKLARHS KFGMKGPASV ISNDDDSASP LHHISNGSNT PSSSEGGPDA
VIIGMTKIPV IENPQYFGIT NSQLKPDTFV QHIKRHNIVL KRELGEGAFG
KVFLAECYNL CPEQDKILVA VKTLKDASDN ARKDFHREAE LLTNLQHEHI
VKFYGVCVEG DPLIMVFEYM KHGDLNKFLR AHGPDAVLMA EGNPPTELTQ
SQMLHIAQQI AAGMVYLASQ HFVHRDLATR NCLVGENLLV KIGDFGMSRD
VYSTDYYRVG GHTMLPIRWM PPESIMYRKF TTESDVWSLG VVLWEIFTYG
KQPWYQLSNN EVIECITQGR VLQRPRTCPQ EVYELMLGCW QREPHMRKNI
KGIHTLLQNL AKASPVYLDI LG
UCSC uc004anz.1; human. [Q16620-1];

Category

Human NTRK2 ELISA Kit